Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa

György Botond Kiss, Éva Vincze, Zoltán Végh, Gábor Tóth, József Soós

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A new nodule-specific gene, Nms-25 (nodulin-25), was identified in cDNA clones isolated from a nodule-specific cDNA library of Medicago sativa. The first transcript of this gene appeared 9 days after inoculation of the roots with Rhizobium meliloti. The time of expression and the quantity of the transcripts of the Nms-25 gene was similar to that of leghemoglobin genes suggesting a similar regulation. A protein of 246 amino acids could be deduced from a full-length cDNA clone. The first 24 amino acids at the N-terminal end of this protein formed a signal sequence which might direct membrane transport into the peribacteroid space. Using different predictive methods the signal sequence cleaved protein was tentatively predicted to be a water-soluble enzyme, but not hydrolase.

Original languageEnglish
Pages (from-to)467-475
Number of pages9
JournalPlant molecular biology
Issue number4
Publication statusPublished - Apr 1 1990



  • Nms-25 (nodulin-25) DNA sequence
  • alfalfa
  • nodule-specific expression
  • protein secondary structure
  • signal peptide
  • symbiotic nitrogen fixation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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