Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa

G. Kiss, Éva Vincze, Zoltán Végh, Gábor Tóth, József Soós

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A new nodule-specific gene, Nms-25 (nodulin-25), was identified in cDNA clones isolated from a nodule-specific cDNA library of Medicago sativa. The first transcript of this gene appeared 9 days after inoculation of the roots with Rhizobium meliloti. The time of expression and the quantity of the transcripts of the Nms-25 gene was similar to that of leghemoglobin genes suggesting a similar regulation. A protein of 246 amino acids could be deduced from a full-length cDNA clone. The first 24 amino acids at the N-terminal end of this protein formed a signal sequence which might direct membrane transport into the peribacteroid space. Using different predictive methods the signal sequence cleaved protein was tentatively predicted to be a water-soluble enzyme, but not hydrolase.

Original languageEnglish
Pages (from-to)467-475
Number of pages9
JournalPlant Molecular Biology
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 1990

Fingerprint

Medicago sativa
Cloning
Organism Cloning
molecular cloning
Complementary DNA
Genes
Protein Sorting Signals
signal peptide
Leghemoglobin
genes
Clone Cells
clones
root inoculation
Sinorhizobium meliloti
leghemoglobin
Amino Acids
Ensifer meliloti
Proteins
amino acids
proteins

Keywords

  • alfalfa
  • Nms-25 (nodulin-25) DNA sequence
  • nodule-specific expression
  • protein secondary structure
  • signal peptide
  • symbiotic nitrogen fixation

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa. / Kiss, G.; Vincze, Éva; Végh, Zoltán; Tóth, Gábor; Soós, József.

In: Plant Molecular Biology, Vol. 14, No. 4, 04.1990, p. 467-475.

Research output: Contribution to journalArticle

Kiss, G. ; Vincze, Éva ; Végh, Zoltán ; Tóth, Gábor ; Soós, József. / Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa. In: Plant Molecular Biology. 1990 ; Vol. 14, No. 4. pp. 467-475.
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