ID sequence-binding protein factor complexed in ribonucleoprotein particles

Kaijiro Anzal, S. Goto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We analyzed brain extracts from fetal or adult mice with a 32P-labeled 5′-half fragment of identifier (ID) sequence in a gel mobility shift assay. Upon digestion of extracts with RNase A prior to the binding reactions, a protein factor(s) that specifically binds to the fragment was shown to appreciably increase in amount with either of the extracts. Furthermore, the binding was competed with single-stranded ID sequences. These observations suggest that the protein factor is capable of interacting with either the genes or their small RNA transcripts.

Original languageEnglish
Pages (from-to)246-250
Number of pages5
JournalNeuroscience Letters
Volume89
Issue number2
DOIs
Publication statusPublished - Jun 29 1988

Fingerprint

Ribonucleoproteins
Carrier Proteins
Pancreatic Ribonuclease
Electrophoretic Mobility Shift Assay
Digestion
Proteins
Gels
RNA
Brain
Genes

Keywords

  • Deoxyribonucleic acid-binding protein
  • Development
  • Electrophoretic mobility shift assay
  • Identifier sequence
  • Mouse brain
  • Ribonucleoprotein particle

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

ID sequence-binding protein factor complexed in ribonucleoprotein particles. / Anzal, Kaijiro; Goto, S.

In: Neuroscience Letters, Vol. 89, No. 2, 29.06.1988, p. 246-250.

Research output: Contribution to journalArticle

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