The concentration of some trace elements of clinical importance was studied in blood serum samples of healthy subjects (n=19, mean age 22±4.5 years) to determine reference ranges for the healthy urban population in Eastern Hungary. A sample preparation method has been developed using closed-vessel microwave digestion with an oxidizing acid mixture (nitric acid and hydrogen peroxide) for multielemental analysis and atmospheric wet digestion with the aqueous solution of trimethyl amine (TMAH) for the measurement of aluminum. Contamination of the samples with Al was observed during acidic microwave digestion because of the chemicals used. Trace element concentrations in the samples were measured with ICP-MS. The reference ranges obtained were (in μg/L): Al 0.98-1.74, Cr 42.8-59.3, Mn 2.27-5.05, Fe 1282-2050, Co 0.22-0.88, Ni 0.03-16.33, Cu 691-1003, Zn 591-1217, Sr 30.37-47.37, Mo 0.73-1.19, Cd 0.02-0.62, and Pb 0.02-2.70. The results were compared to the reference ranges actually used in the clinical practice, some of which are available in the literature. The effect of the applied digestion methods on the obtained individual results and average concentration ranges was also studied.
|Number of pages||5|
|Publication status||Published - Jan 1 2001|
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