ICP-MS-assisted nanoHPLC-electrospray Q/time-of-flight MS/MS selenopeptide mapping in Brazil nuts

M. Dernovics, Pierre Giusti, Ryszard Lobinski

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A method was developed for the identification of selenium-containing peptides issued from proteins of a Se-rich (82.9 mg kg-1) foodstuff, Brazil nut (Bertholletia excelsa). A sample purification procedure was optimized to cope with the 100-fold excess of sulfur analogues and matrix interferences. It was based on the consecutive size-exclusion fractionation of proteins and tryptic peptides, and enrichment of the Se-containing fractions, prior to nanoHPLC-ES-Q/TOF MS/MS. The characteristic isotopic patterns of selenium compounds (always minor peaks) were detected in ESI mass spectra at retention times precisely indicated by the matrix interference-free, sensitive (DL 1.3 fmol) 80Se detection by ICP collision cell MS in the same separation conditions. The potential of the method was demonstrated by the identification of 15 Se-containing peptides, from which all but one were found to originate from the selenised isoforms of the 2S protein.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalJournal of Analytical Atomic Spectrometry
Volume22
Issue number1
DOIs
Publication statusPublished - 2007

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Peptides
Selenium Compounds
Proteins
Fractionation
Selenium
Sulfur
Purification
Protein Isoforms

ASJC Scopus subject areas

  • Spectroscopy

Cite this

ICP-MS-assisted nanoHPLC-electrospray Q/time-of-flight MS/MS selenopeptide mapping in Brazil nuts. / Dernovics, M.; Giusti, Pierre; Lobinski, Ryszard.

In: Journal of Analytical Atomic Spectrometry, Vol. 22, No. 1, 2007, p. 41-50.

Research output: Contribution to journalArticle

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