ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells

Peter Nagy, G. Vámosi, S. Damjanovich, László Damjanovich

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

ICAM-1 and MHC-I proteins play fundamental roles in antigen presentation, activation of T lymphocytes, and immune responses against tumor cells. Both of them participate in the formation of lipid raft-associated membrane protein clusters. We found significant colocalization between ICAM-1 and MHC-I at the level of large-scale associations. We combined RNA interference and fluorescence resonance energy transfer studies to show that ICAM-1 promotes the partial disassembly of MHC-I homoclusters on LS-174T colon carcinoma cells. Interferon-γ (IFN-γ) treatment induced an increase in the expression of MHC-I and ICAM-1 resulting in decreased MHC-I homoassociation. Small interfering RNAs directed against ICAM-1 restored the homoassociation of MHC-I without influencing the expression level of MHC-I by eliminating ICAM-1 molecules interspersed in MHC-I clusters. We conclude that the composition of membrane protein clusters is dynamically altered in response to both physiological and experimentally elicited changes in antigen expression levels.

Original languageEnglish
Pages (from-to)758-763
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume347
Issue number3
DOIs
Publication statusPublished - Sep 1 2006

Fingerprint

Intercellular Adhesion Molecule-1
Colon
Cells
Carcinoma
Membrane Proteins
Antigens
Fluorescence Resonance Energy Transfer
T-cells
Antigen Presentation
RNA Interference
Interferons
Small Interfering RNA
Tumors
Chemical activation
RNA
T-Lymphocytes
Lipids
Molecules
Chemical analysis
Neoplasms

Keywords

  • Colon carcinoma
  • Confocal microscopy
  • Flow cytometry
  • FRET
  • ICAM-1
  • Membrane protein association
  • MHC-I
  • Receptor clustering
  • RNA interference

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells. / Nagy, Peter; Vámosi, G.; Damjanovich, S.; Damjanovich, László.

In: Biochemical and Biophysical Research Communications, Vol. 347, No. 3, 01.09.2006, p. 758-763.

Research output: Contribution to journalArticle

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