Hypoxia-inducible factor 1-alpha release after intracoronary versus intramyocardial stem cell therapy in myocardial infarction

Mariann Gyöngyösi, Rayyan Hemetsberger, A. Pósa, Silvia Charwat, Noemi Pavo, Örs Petnehazy, Zsolt Petrasi, Imre J. Pavo, Hani Hemetsberger, Imre Benedek, Teodora Benedek, Istvan Benedek, Istvan Kovacs, Christoph Kaun, Gerald Maurer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have investigated the effect of stem cell delivery on the release of hypoxia-inducible factor 1 alpha (HIF-1α) in peripheral circulation and myocardium in experimental myocardial ischemia. Closed-chest, reperfused myocardial infarction (MI) was created in domestic pigs. Porcine mesenchymal stem cells (MSCs) were cultured and delivered (9.8±1.2×10 6) either percutaneously NOGAguided transendocardially (Group IM) or intracoronary (Group IC) 22±4 days post-MI. Pigs without MSC delivery served as sham control (Group S). Plasma HIF-1α was measured at baseline, immediately post-and at follow-up (FUP; 2 h or 24 h) post-MSC delivery by ELISA kit. Myocardial HIF-1α expression of infarcted, normal myocardium, or border zone was determined by Western blot. Plasma level of HIF-1α increased immediately post-MI (from 278±127 to 631±375 pg/ml, p

Original languageEnglish
Pages (from-to)114-121
Number of pages8
JournalJournal of Cardiovascular Translational Research
Volume3
Issue number2
DOIs
Publication statusPublished - 2010

Fingerprint

Hypoxia-Inducible Factor 1
Cell- and Tissue-Based Therapy
Stem Cells
Mesenchymal Stromal Cells
Myocardial Infarction
Myocardium
Swine
Sus scrofa
Myocardial Ischemia
Thorax
Western Blotting
Enzyme-Linked Immunosorbent Assay
Control Groups

Keywords

  • Cardiac delivery
  • Hypoxia-inducible factor 1 alpha
  • Myocardial infarction
  • Stem cell

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Genetics
  • Genetics(clinical)
  • Molecular Medicine
  • Pharmaceutical Science
  • Medicine(all)

Cite this

Hypoxia-inducible factor 1-alpha release after intracoronary versus intramyocardial stem cell therapy in myocardial infarction. / Gyöngyösi, Mariann; Hemetsberger, Rayyan; Pósa, A.; Charwat, Silvia; Pavo, Noemi; Petnehazy, Örs; Petrasi, Zsolt; Pavo, Imre J.; Hemetsberger, Hani; Benedek, Imre; Benedek, Teodora; Benedek, Istvan; Kovacs, Istvan; Kaun, Christoph; Maurer, Gerald.

In: Journal of Cardiovascular Translational Research, Vol. 3, No. 2, 2010, p. 114-121.

Research output: Contribution to journalArticle

Gyöngyösi, M, Hemetsberger, R, Pósa, A, Charwat, S, Pavo, N, Petnehazy, Ö, Petrasi, Z, Pavo, IJ, Hemetsberger, H, Benedek, I, Benedek, T, Benedek, I, Kovacs, I, Kaun, C & Maurer, G 2010, 'Hypoxia-inducible factor 1-alpha release after intracoronary versus intramyocardial stem cell therapy in myocardial infarction', Journal of Cardiovascular Translational Research, vol. 3, no. 2, pp. 114-121. https://doi.org/10.1007/s12265-009-9154-1
Gyöngyösi, Mariann ; Hemetsberger, Rayyan ; Pósa, A. ; Charwat, Silvia ; Pavo, Noemi ; Petnehazy, Örs ; Petrasi, Zsolt ; Pavo, Imre J. ; Hemetsberger, Hani ; Benedek, Imre ; Benedek, Teodora ; Benedek, Istvan ; Kovacs, Istvan ; Kaun, Christoph ; Maurer, Gerald. / Hypoxia-inducible factor 1-alpha release after intracoronary versus intramyocardial stem cell therapy in myocardial infarction. In: Journal of Cardiovascular Translational Research. 2010 ; Vol. 3, No. 2. pp. 114-121.
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