Hypotonic stress influence the membrane potential and alter the proliferation of keratinocytes in vitro

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Abstract

Keratinocyte proliferation and differentiation is strongly influenced by mechanical forces. We investigated the effect of osmotic changes in the development of HaCaT cells in culture using intracellular calcium measurements, electrophysiological recordings and molecular biology techniques. The application of hypotonic stress (174mOsmol/l) caused a sustained hyperpolarization of HaCaT cells from a resting potential of -27±4 to -51±9mV. This change was partially reversible. The surface membrane channels involved in the hyperpolarization were identified as chloride channels due to the lack of response in the absence of the anion. Cells responded with an elevation of intracellular calcium concentration to hypotonic stress, which critically depended on external calcium. The presence of phorbol-12-myristate-13-acetate in the culture medium for 12h augmented the subsequent response to hypotonic stress. A sudden switch from iso- to hypotonic solution increased cell proliferation and suppressed the production of involucrin, filaggrin and transglutaminase, markers of keratinocyte differentiation. It is concluded that sudden mechanical forces increase the proliferation of keratinocytes through alterations in their membrane potential and intracellular calcium concentration. These changes together with additional modifications in channel expression and intracellular signalling mechanisms could underlie the increased proliferation of keratinocytes in hyperproliferative skin diseases.

Original languageEnglish
Pages (from-to)302-310
Number of pages9
JournalExperimental Dermatology
Volume16
Issue number4
DOIs
Publication statusPublished - Apr 2007

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Osmotic Pressure
Keratinocytes
Membrane Potentials
Calcium
Membranes
Hypotonic Solutions
Chloride Channels
Molecular biology
Transglutaminases
Differentiation Antigens
Cell proliferation
Ion Channels
Skin Diseases
Anions
Culture Media
Molecular Biology
Skin
Acetates
Cell Culture Techniques
Switches

Keywords

  • Chloride current
  • Intracellular calcium concentration
  • Keratinocytes
  • Proliferation
  • Protein kinase C

ASJC Scopus subject areas

  • Dermatology

Cite this

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title = "Hypotonic stress influence the membrane potential and alter the proliferation of keratinocytes in vitro",
abstract = "Keratinocyte proliferation and differentiation is strongly influenced by mechanical forces. We investigated the effect of osmotic changes in the development of HaCaT cells in culture using intracellular calcium measurements, electrophysiological recordings and molecular biology techniques. The application of hypotonic stress (174mOsmol/l) caused a sustained hyperpolarization of HaCaT cells from a resting potential of -27±4 to -51±9mV. This change was partially reversible. The surface membrane channels involved in the hyperpolarization were identified as chloride channels due to the lack of response in the absence of the anion. Cells responded with an elevation of intracellular calcium concentration to hypotonic stress, which critically depended on external calcium. The presence of phorbol-12-myristate-13-acetate in the culture medium for 12h augmented the subsequent response to hypotonic stress. A sudden switch from iso- to hypotonic solution increased cell proliferation and suppressed the production of involucrin, filaggrin and transglutaminase, markers of keratinocyte differentiation. It is concluded that sudden mechanical forces increase the proliferation of keratinocytes through alterations in their membrane potential and intracellular calcium concentration. These changes together with additional modifications in channel expression and intracellular signalling mechanisms could underlie the increased proliferation of keratinocytes in hyperproliferative skin diseases.",
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AU - Gönczi, M.

AU - Szentandrássy, N.

AU - Fülöp, L.

AU - Telek, Andrea

AU - Szigeti, G.

AU - Magyar, J.

AU - Bíró, T.

AU - Nánási, P.

AU - Csernoch, L.

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