Hypomethylation of the decorin proteoglycan gene in human colon cancer

R. Ádány, R. V. Iozzo

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We have previously reported that the connective tissue stroma of human colon carcinoma contains elevated amounts of decorin, a small proteoglycan involved in the regulation of matrix formation and cell proliferation. These biochemical changes were correlated with increased mRNA levels and general hypomethylation of the decorin gene in human colon cancer DNA. In this report we use a quantitative polymerase chain reaction method coupled with digestion of the DNA template by methylation-sensitive restriction endonucleases to investigate in detail the location of hypomethylated sites in decorin gene. We demonstrate that a specific site in the 3' region of the gene, encompassing codons 360-361, is specifically hypomethylated in both colon carcinoma and benign polyp. In contrast, three HpaII sites, clustered in the 5' untranslated region, show full methylation in normal and neoplastic DNA. The lack of such changes in ulcerative colitis DNA suggests that chronic inflammation alone is not sufficient to alter cytosine methylation in the decorin gene. These results suggest the possibility that the 3' region of the decorin-coding sequence may be involved in the control of decorin gene expression.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalBiochemical Journal
Volume276
Issue number2
Publication statusPublished - 1991

Fingerprint

Decorin
Proteoglycans
Colonic Neoplasms
Genes
Methylation
DNA
Colon
Carcinoma
5' Untranslated Regions
Polymerase chain reaction
Cytosine
DNA Restriction Enzymes
Cell proliferation
DNA Methylation
Polyps
Ulcerative Colitis
Gene expression
Codon
Connective Tissue
Digestion

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hypomethylation of the decorin proteoglycan gene in human colon cancer. / Ádány, R.; Iozzo, R. V.

In: Biochemical Journal, Vol. 276, No. 2, 1991, p. 301-306.

Research output: Contribution to journalArticle

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