Hypoglossal motor neurons display a reduced calcium increase after axotomy in mice with upregulated parvalbumin

Melinda Paizs, J. Engelhardt, Zója Katarova, L. Siklós

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Motor neurons that exhibit differences in vulnerability to degeneration have been identified in motor neuron disease and in its animal models. The oculomotor and hypoglossal neurons are regarded as the prototypes of the resistant and susceptible cell types, respectively. Because an increase in the level of intracellular calcium has been proposed as a feature amplifying degenerative processes, we earlier studied the calcium increase in these motor neurons after axotomy in Balb/c mice and demonstrated a correlation between the susceptibility to degeneration and the intracellular calcium increase, with an inverse relation with the calcium buffering capacity, characterized by the parvalbumin or calbindin-D28K content. Because the differential susceptibility of the cells might also be attributed to their different cellular environments, in the present experiments, with the aim of verifying directly that a higher calcium buffering capacity is indeed responsible for the enhanced resistance, motor neurons were studied in their original milieu in mice with a genetically increased parvalbumin level. The changes in intracellular calcium level of the hypoglossal and oculomotor neurons after axotomy were studied electron microscopically at a 21-day interval after axotomy, during which time no significant calcium increase was detected in the hypoglossal motor neurons, the response being similar to that of the oculomotor neurons. The hypoglossal motor neurons of the parental mice, used as positive controls, exhibited a transient, significant elevation of calcium. These data provide more direct evidence of the protective role of parvalbumin against the degeneration mediated by a calcium increase in the acute injury of motor neurons.

Original languageEnglish
Pages (from-to)1946-1961
Number of pages16
JournalJournal of Comparative Neurology
Volume518
Issue number11
DOIs
Publication statusPublished - Jun 1 2010

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Axotomy
Parvalbumins
Motor Neurons
Calcium
Neurons
Calbindin 1
Motor Neuron Disease
Animal Models
Electrons

Keywords

  • Axotomy
  • Calcium
  • Calcium buffering
  • Neuroprotection
  • Parvalbumin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hypoglossal motor neurons display a reduced calcium increase after axotomy in mice with upregulated parvalbumin. / Paizs, Melinda; Engelhardt, J.; Katarova, Zója; Siklós, L.

In: Journal of Comparative Neurology, Vol. 518, No. 11, 01.06.2010, p. 1946-1961.

Research output: Contribution to journalArticle

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