Hypersensitive cell death and papilla formation in barley attacked by the powdery mildew fungus are associated with hydrogen peroxide but not with salicylic acid accumulation

Ralph Hückelhoven, J. Fodor, Christine Preis, Karl Heinz Kogel

Research output: Contribution to journalArticle

289 Citations (Scopus)

Abstract

We analyzed the pathogenesis-related generation of H2O2 using the microscopic detection of 3,3-diaminobenzidine polymerization in near-isogenic barley (Hordeum vulgare L.) lines carrying different powdery mildew (Blumeria graminis f.sp. hordei) resistance genes, and in a line expressing chemically activated resistance after treatment with 2,6-dichloroisonicotinic acid (DCINA). Hypersensitive cell death in Mla12 and Mlg genotypes or after chemical activation by DCINA was associated with H2O2, accumulation throughout attacked cells. Formation of cell wall appositions (papillae) mediated in Mlg and Mlo5 genotypes and in DCINA-activated plants was paralleled by H2O2 accumulation in effective papillae and in cytosolic vesicles of up to 2 μm in diameter near the papillae. H2O2 was not detected in ineffective papillae of cells that had been successfully penetrated by the fungus. These findings support the hypothesis that H2O2 may play a substantial role in plant defense against the powdery mildew fungus. We did not detect any accumulation of salicylic acid in primary leaves after inoculation of the different barley genotypes, indicating that these defense responses neither relied on nor provoked salicylic acid accumulation in barley.

Original languageEnglish
Pages (from-to)1251-1260
Number of pages10
JournalPlant Physiology
Volume119
Issue number4
Publication statusPublished - Apr 1999

Fingerprint

Salicylic Acid
Hordeum
powdery mildew
salicylic acid
Hydrogen Peroxide
hydrogen peroxide
cell death
Fungi
Cell Death
barley
fungi
genotype
acids
Genotype
Blumeria graminis f. sp. hordei
polymerization
Hordeum vulgare
pathogenesis
cell walls
Polymerization

ASJC Scopus subject areas

  • Plant Science

Cite this

Hypersensitive cell death and papilla formation in barley attacked by the powdery mildew fungus are associated with hydrogen peroxide but not with salicylic acid accumulation. / Hückelhoven, Ralph; Fodor, J.; Preis, Christine; Kogel, Karl Heinz.

In: Plant Physiology, Vol. 119, No. 4, 04.1999, p. 1251-1260.

Research output: Contribution to journalArticle

@article{1e3a081cd11c4670a14e2d0d6cd17005,
title = "Hypersensitive cell death and papilla formation in barley attacked by the powdery mildew fungus are associated with hydrogen peroxide but not with salicylic acid accumulation",
abstract = "We analyzed the pathogenesis-related generation of H2O2 using the microscopic detection of 3,3-diaminobenzidine polymerization in near-isogenic barley (Hordeum vulgare L.) lines carrying different powdery mildew (Blumeria graminis f.sp. hordei) resistance genes, and in a line expressing chemically activated resistance after treatment with 2,6-dichloroisonicotinic acid (DCINA). Hypersensitive cell death in Mla12 and Mlg genotypes or after chemical activation by DCINA was associated with H2O2, accumulation throughout attacked cells. Formation of cell wall appositions (papillae) mediated in Mlg and Mlo5 genotypes and in DCINA-activated plants was paralleled by H2O2 accumulation in effective papillae and in cytosolic vesicles of up to 2 μm in diameter near the papillae. H2O2 was not detected in ineffective papillae of cells that had been successfully penetrated by the fungus. These findings support the hypothesis that H2O2 may play a substantial role in plant defense against the powdery mildew fungus. We did not detect any accumulation of salicylic acid in primary leaves after inoculation of the different barley genotypes, indicating that these defense responses neither relied on nor provoked salicylic acid accumulation in barley.",
author = "Ralph H{\"u}ckelhoven and J. Fodor and Christine Preis and Kogel, {Karl Heinz}",
year = "1999",
month = "4",
language = "English",
volume = "119",
pages = "1251--1260",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

TY - JOUR

T1 - Hypersensitive cell death and papilla formation in barley attacked by the powdery mildew fungus are associated with hydrogen peroxide but not with salicylic acid accumulation

AU - Hückelhoven, Ralph

AU - Fodor, J.

AU - Preis, Christine

AU - Kogel, Karl Heinz

PY - 1999/4

Y1 - 1999/4

N2 - We analyzed the pathogenesis-related generation of H2O2 using the microscopic detection of 3,3-diaminobenzidine polymerization in near-isogenic barley (Hordeum vulgare L.) lines carrying different powdery mildew (Blumeria graminis f.sp. hordei) resistance genes, and in a line expressing chemically activated resistance after treatment with 2,6-dichloroisonicotinic acid (DCINA). Hypersensitive cell death in Mla12 and Mlg genotypes or after chemical activation by DCINA was associated with H2O2, accumulation throughout attacked cells. Formation of cell wall appositions (papillae) mediated in Mlg and Mlo5 genotypes and in DCINA-activated plants was paralleled by H2O2 accumulation in effective papillae and in cytosolic vesicles of up to 2 μm in diameter near the papillae. H2O2 was not detected in ineffective papillae of cells that had been successfully penetrated by the fungus. These findings support the hypothesis that H2O2 may play a substantial role in plant defense against the powdery mildew fungus. We did not detect any accumulation of salicylic acid in primary leaves after inoculation of the different barley genotypes, indicating that these defense responses neither relied on nor provoked salicylic acid accumulation in barley.

AB - We analyzed the pathogenesis-related generation of H2O2 using the microscopic detection of 3,3-diaminobenzidine polymerization in near-isogenic barley (Hordeum vulgare L.) lines carrying different powdery mildew (Blumeria graminis f.sp. hordei) resistance genes, and in a line expressing chemically activated resistance after treatment with 2,6-dichloroisonicotinic acid (DCINA). Hypersensitive cell death in Mla12 and Mlg genotypes or after chemical activation by DCINA was associated with H2O2, accumulation throughout attacked cells. Formation of cell wall appositions (papillae) mediated in Mlg and Mlo5 genotypes and in DCINA-activated plants was paralleled by H2O2 accumulation in effective papillae and in cytosolic vesicles of up to 2 μm in diameter near the papillae. H2O2 was not detected in ineffective papillae of cells that had been successfully penetrated by the fungus. These findings support the hypothesis that H2O2 may play a substantial role in plant defense against the powdery mildew fungus. We did not detect any accumulation of salicylic acid in primary leaves after inoculation of the different barley genotypes, indicating that these defense responses neither relied on nor provoked salicylic acid accumulation in barley.

UR - http://www.scopus.com/inward/record.url?scp=0037786127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037786127&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0037786127

VL - 119

SP - 1251

EP - 1260

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -