Hyperacute serum has markedly better regenerative efficacy than platelet-rich plasma in a human bone oxygen-glucose deprivation model

Gabriella Vácz, Bálint Major, Dorottya Gaál, Laura Petrik, Dénes Balázs Horváthy, Weiping Han, Tünde Holczer, Melinda Simon, Jeffrey M. Muir, István Hornyák, Z. Lacza

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Abstract

Aim: Platelet-rich plasma (PRP) and hyperacute serum (HAS) were compared in a novel human model of ex vivo bone damage induced by oxygen-glucose deprivation (OGD). Materials & methods: Osteoarthritic subchondral bone pieces were harvested from discarded femoral heads during hip replacement surgery and subjected to transient OGD. Results: Proteome profiling revealed that PRP is more angiopoietic, whereas HAS is more antiangiopoietic in composition. However, treatment of OGD-exposed bone with multiple PRP preparations had no effect on cell counts, whereas HAS restored cell proliferation capacity and rescued viable cell number following OGD. Conclusion: A similar pro-proliferation effect was observed with recombinant growth factors, indicating that HAS may be an alternative agent for enhancing the regeneration of damaged bone cells.

Original languageEnglish
Pages (from-to)531-543
Number of pages13
JournalRegenerative Medicine
Volume13
Issue number5
DOIs
Publication statusPublished - Jan 1 2018

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Keywords

  • blood fractions
  • bone necrosis
  • femoral head
  • growth factors
  • ischemia

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

Cite this

Vácz, G., Major, B., Gaál, D., Petrik, L., Horváthy, D. B., Han, W., Holczer, T., Simon, M., Muir, J. M., Hornyák, I., & Lacza, Z. (2018). Hyperacute serum has markedly better regenerative efficacy than platelet-rich plasma in a human bone oxygen-glucose deprivation model. Regenerative Medicine, 13(5), 531-543. https://doi.org/10.2217/rme-2017-0141