Hygromycin B, carboxin and nourseothricin susceptibility of polyunsaturated fatty acid producing Mortierella and Umbelopsis strains

Ildikó Nyilasi, Kata E. Kristó, Bettina Pálffy, Márta Hegyi, Muthusamy Chandrasekaran, Shine Kadaikunnan, Naiyf S. Alharbi, T. Papp, C. Vágvölgyi

Research output: Contribution to journalArticle

Abstract

Mortierella and Umbelopsis species are particularly active in polyunsaturated fatty acid (PUFA) synthesis as they are able to produce many ω-3 and ω-6 PUFAs. Genetic manipulation of the lipid production to generate PUFA overproducing strains and strains with altered PUFA profile requires well-established transformation systems and reliable selectable markers. Therefore, we screened different antifungal agents, which can be used for selection in further transformation experiments. Hygromycin B, carboxin, pyrithiamine and nourseothricin susceptibility of several Mortierella and Umbelopsis isolates was investigated using a broth microdilution method. Pyrithiamine was totally ineffective against all isolates while the other three antifungal agents were active against Mortierella and Umbelopsis strains. Several Mortierella isolates represented high sensitivity to hygromycin B whilst nourseothricin was rather active against Umbelopsis species. Carboxin inhibited the hyphal growth and the spore germination of all isolates completely in low concentrations.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalActa Biologica Szegediensis
Volume59
Issue number1
Publication statusPublished - 2015

Fingerprint

Carboxin
Umbelopsis
Streptothricins
Mortierella
Hygromycin B
carboxin
hygromycin B
Pyrithiamine
Unsaturated Fatty Acids
polyunsaturated fatty acids
pyrithiamine
Antifungal Agents
antifungal agents
spore germination
Germination
minimum inhibitory concentration
Spores
genetic engineering
Lipids
fatty acid composition

Keywords

  • Carboxin
  • Hygromycin B
  • Mortierella
  • Nourseothricin
  • Polyunsaturated fatty acid
  • Umbelopsis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hygromycin B, carboxin and nourseothricin susceptibility of polyunsaturated fatty acid producing Mortierella and Umbelopsis strains. / Nyilasi, Ildikó; Kristó, Kata E.; Pálffy, Bettina; Hegyi, Márta; Chandrasekaran, Muthusamy; Kadaikunnan, Shine; Alharbi, Naiyf S.; Papp, T.; Vágvölgyi, C.

In: Acta Biologica Szegediensis, Vol. 59, No. 1, 2015, p. 11-18.

Research output: Contribution to journalArticle

Nyilasi, I, Kristó, KE, Pálffy, B, Hegyi, M, Chandrasekaran, M, Kadaikunnan, S, Alharbi, NS, Papp, T & Vágvölgyi, C 2015, 'Hygromycin B, carboxin and nourseothricin susceptibility of polyunsaturated fatty acid producing Mortierella and Umbelopsis strains', Acta Biologica Szegediensis, vol. 59, no. 1, pp. 11-18.
Nyilasi, Ildikó ; Kristó, Kata E. ; Pálffy, Bettina ; Hegyi, Márta ; Chandrasekaran, Muthusamy ; Kadaikunnan, Shine ; Alharbi, Naiyf S. ; Papp, T. ; Vágvölgyi, C. / Hygromycin B, carboxin and nourseothricin susceptibility of polyunsaturated fatty acid producing Mortierella and Umbelopsis strains. In: Acta Biologica Szegediensis. 2015 ; Vol. 59, No. 1. pp. 11-18.
@article{f2664d3fa4184ef4b9e03f090447d237,
title = "Hygromycin B, carboxin and nourseothricin susceptibility of polyunsaturated fatty acid producing Mortierella and Umbelopsis strains",
abstract = "Mortierella and Umbelopsis species are particularly active in polyunsaturated fatty acid (PUFA) synthesis as they are able to produce many ω-3 and ω-6 PUFAs. Genetic manipulation of the lipid production to generate PUFA overproducing strains and strains with altered PUFA profile requires well-established transformation systems and reliable selectable markers. Therefore, we screened different antifungal agents, which can be used for selection in further transformation experiments. Hygromycin B, carboxin, pyrithiamine and nourseothricin susceptibility of several Mortierella and Umbelopsis isolates was investigated using a broth microdilution method. Pyrithiamine was totally ineffective against all isolates while the other three antifungal agents were active against Mortierella and Umbelopsis strains. Several Mortierella isolates represented high sensitivity to hygromycin B whilst nourseothricin was rather active against Umbelopsis species. Carboxin inhibited the hyphal growth and the spore germination of all isolates completely in low concentrations.",
keywords = "Carboxin, Hygromycin B, Mortierella, Nourseothricin, Polyunsaturated fatty acid, Umbelopsis",
author = "Ildik{\'o} Nyilasi and Krist{\'o}, {Kata E.} and Bettina P{\'a}lffy and M{\'a}rta Hegyi and Muthusamy Chandrasekaran and Shine Kadaikunnan and Alharbi, {Naiyf S.} and T. Papp and C. V{\'a}gv{\"o}lgyi",
year = "2015",
language = "English",
volume = "59",
pages = "11--18",
journal = "Acta Biologica Szegediensis",
issn = "1588-385X",
publisher = "University of Szeged",
number = "1",

}

TY - JOUR

T1 - Hygromycin B, carboxin and nourseothricin susceptibility of polyunsaturated fatty acid producing Mortierella and Umbelopsis strains

AU - Nyilasi, Ildikó

AU - Kristó, Kata E.

AU - Pálffy, Bettina

AU - Hegyi, Márta

AU - Chandrasekaran, Muthusamy

AU - Kadaikunnan, Shine

AU - Alharbi, Naiyf S.

AU - Papp, T.

AU - Vágvölgyi, C.

PY - 2015

Y1 - 2015

N2 - Mortierella and Umbelopsis species are particularly active in polyunsaturated fatty acid (PUFA) synthesis as they are able to produce many ω-3 and ω-6 PUFAs. Genetic manipulation of the lipid production to generate PUFA overproducing strains and strains with altered PUFA profile requires well-established transformation systems and reliable selectable markers. Therefore, we screened different antifungal agents, which can be used for selection in further transformation experiments. Hygromycin B, carboxin, pyrithiamine and nourseothricin susceptibility of several Mortierella and Umbelopsis isolates was investigated using a broth microdilution method. Pyrithiamine was totally ineffective against all isolates while the other three antifungal agents were active against Mortierella and Umbelopsis strains. Several Mortierella isolates represented high sensitivity to hygromycin B whilst nourseothricin was rather active against Umbelopsis species. Carboxin inhibited the hyphal growth and the spore germination of all isolates completely in low concentrations.

AB - Mortierella and Umbelopsis species are particularly active in polyunsaturated fatty acid (PUFA) synthesis as they are able to produce many ω-3 and ω-6 PUFAs. Genetic manipulation of the lipid production to generate PUFA overproducing strains and strains with altered PUFA profile requires well-established transformation systems and reliable selectable markers. Therefore, we screened different antifungal agents, which can be used for selection in further transformation experiments. Hygromycin B, carboxin, pyrithiamine and nourseothricin susceptibility of several Mortierella and Umbelopsis isolates was investigated using a broth microdilution method. Pyrithiamine was totally ineffective against all isolates while the other three antifungal agents were active against Mortierella and Umbelopsis strains. Several Mortierella isolates represented high sensitivity to hygromycin B whilst nourseothricin was rather active against Umbelopsis species. Carboxin inhibited the hyphal growth and the spore germination of all isolates completely in low concentrations.

KW - Carboxin

KW - Hygromycin B

KW - Mortierella

KW - Nourseothricin

KW - Polyunsaturated fatty acid

KW - Umbelopsis

UR - http://www.scopus.com/inward/record.url?scp=84937510903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937510903&partnerID=8YFLogxK

M3 - Article

VL - 59

SP - 11

EP - 18

JO - Acta Biologica Szegediensis

JF - Acta Biologica Szegediensis

SN - 1588-385X

IS - 1

ER -