Hydroperoxide-induced nucleotide degradation and adenosine formation in isolated rat cardiomyocytes

Bo S. Andersson, Ricardo F. Vidal, Monica Sundberg, Jovan Rajs, P. Sótónyi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of tert-butyl hydroperoxide (tBH) on the nucleotide level in isolated cardiomyocytes from adult rats was examined. Incubation of myocytes with the hydroperoxide resulted in loss of both ATP and NAD from mitochondria and cytosol. In contrast, only the cytosolic level of NADP was increased. The effect on myocyte content of ADP and AMP was less pronounced. A significant increase in formation and release of adenosine also occurred in tBH-exposed cells. Treatment of cardiomyocytes with tBH resulted in a progressive decline of rod-shaped and viable cells, which was accompanied by an increase in numbers of hypercontracted and round myocytes in the incubation mixture. Electron microscopic examination showed an intense contraction of the cells. In summary our results demonstrate that plasma membrane damage and hypercontraction are early manifestations of tBH-induced injury to isolated cardiomyocytes. The depletion of ATP and formation of adenosine also suggest that degradation of adenine nucleotides may contribute to the cytotoxicity caused by tBH. Possible mechanisms involved in the ATP depletion are discussed.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalToxicology
Volume106
Issue number1-3
DOIs
Publication statusPublished - Jan 8 1996

Fingerprint

tert-Butylhydroperoxide
Cardiac Myocytes
Adenosine
Hydrogen Peroxide
Rats
Nucleotides
Degradation
Muscle Cells
Adenosine Triphosphate
Mitochondria
Adenine Nucleotides
Adenosine Monophosphate
Cell membranes
Cytotoxicity
NADP
NAD
Cytosol
Adenosine Diphosphate
Microscopic examination
Cell Membrane

Keywords

  • Adenine nucleotide degradation
  • Cytotoxicity
  • Electron microscopy
  • Isolated cardiomyocytes
  • Oxidant stress
  • Tert-Butyl hydroperoxide

ASJC Scopus subject areas

  • Toxicology

Cite this

Hydroperoxide-induced nucleotide degradation and adenosine formation in isolated rat cardiomyocytes. / Andersson, Bo S.; Vidal, Ricardo F.; Sundberg, Monica; Rajs, Jovan; Sótónyi, P.

In: Toxicology, Vol. 106, No. 1-3, 08.01.1996, p. 39-48.

Research output: Contribution to journalArticle

Andersson, Bo S. ; Vidal, Ricardo F. ; Sundberg, Monica ; Rajs, Jovan ; Sótónyi, P. / Hydroperoxide-induced nucleotide degradation and adenosine formation in isolated rat cardiomyocytes. In: Toxicology. 1996 ; Vol. 106, No. 1-3. pp. 39-48.
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