Hydrolysis efficiency and enzyme adsorption on steam-pretreated spruce in the presence of poly(ethylene glycol)

Bálint Sipos, Dóra Dienes, Áron Schleicher, Raffaella Perazzini, Claudia Crestini, Matti Siika-aho, Kati Réczey

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Enzymatic hydrolysis of lignocellulosic substrates is one of the limiting steps in second generation bio-ethanol production. The effects of addition of poly(ethylene glycol) 4000 (PEG) on the efficiency and adsorption of cellulase components during the 72-h hydrolysis of steam-pretreated spruce were investigated. It was found that addition of PEG increased the amount of the enzyme components in the hydrolysis supernatant to varying degrees. As an effect of PEG addition the concentration of free proteins increased by 36% and the free overall cellulolytic activity (FPA) in the liquid fraction by 51%. After 72-h hydrolysis with PEG 48-68% higher activities towards 4-methylumbelliferyl-saccharide substrates were recovered. The results suggest that the effect of PEG is based on the decrease of non-productive enzyme binding. The structure of the hydrolysis residues was studied by 31P NMR.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalEnzyme and Microbial Technology
Volume47
Issue number3
DOIs
Publication statusPublished - Aug 1 2010

Keywords

  • Cellulase
  • Enzyme adsorption
  • Hydrolysis
  • Lignocellulose
  • Poly(ethylene glycol)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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