Hydrogen/deuterium exchange of electrosprayed ions in the atmospheric interface of a commercial triple-quadrupole mass spectrometer

Zoltán Takáts, G. Schlosser, K. Vékey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel H/D exchange technique capable of the deuteration of electrosprayed ions has been developed. H/D exchange was carried out by introducing deuterating agent (e.g., d-MeOH) into the curtain gas flow of a commercial triple quadrupole mass spectrometer. In contrast to the widely used H/D exchange techniques, the ions are not trapped in this case. The main advantages of this technique are the ease of use and applicability on most commercial mass spectrometers, including quadrupole-type instruments. Effect of various instrumental parameters was investigated in detail, including spray voltage, spray position, partial pressure of the deuterating agent in the curtain gas, gas flow rates, the orifice-to-skimmer potential and the source temperature. Among these only the partial pressure of the deuterating agent in curtain gas, orifice-to-skimmer potential and source temperature influenced the efficiency of H/D exchange. These suggest that the H/D exchange is likely to occur in the fore-vacuum region of the atmospheric interface. Analytical capabilities of the technique were demonstrated by differentiation of lysine and glutamine protonated molecular ions. Selective quantitation of lysine and glutamine mixtures was achieved, with a lower limit of detection of 1.5% for glutamine and 0.2% for lysine. H/D exchange of multiply charged macromolecular ions can also be carried out using this technique, which was demonstrated using cytochrome c.

Original languageEnglish
Pages (from-to)729-741
Number of pages13
JournalInternational Journal of Mass Spectrometry
Volume228
Issue number2-3
DOIs
Publication statusPublished - Aug 15 2003

Fingerprint

glutamine
curtains
lysine
Deuterium
Mass spectrometers
mass spectrometers
deuterium
Hydrogen
Ion exchange
quadrupoles
Glutamine
orifices
Ions
Lysine
gas flow
sprayers
partial pressure
hydrogen
Orifices
Partial pressure

Keywords

  • Electrospray
  • H/D exchange
  • Ion-molecule reactions

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy

Cite this

@article{e2159ee068844012ba25c16f2b5d8ad6,
title = "Hydrogen/deuterium exchange of electrosprayed ions in the atmospheric interface of a commercial triple-quadrupole mass spectrometer",
abstract = "A novel H/D exchange technique capable of the deuteration of electrosprayed ions has been developed. H/D exchange was carried out by introducing deuterating agent (e.g., d-MeOH) into the curtain gas flow of a commercial triple quadrupole mass spectrometer. In contrast to the widely used H/D exchange techniques, the ions are not trapped in this case. The main advantages of this technique are the ease of use and applicability on most commercial mass spectrometers, including quadrupole-type instruments. Effect of various instrumental parameters was investigated in detail, including spray voltage, spray position, partial pressure of the deuterating agent in the curtain gas, gas flow rates, the orifice-to-skimmer potential and the source temperature. Among these only the partial pressure of the deuterating agent in curtain gas, orifice-to-skimmer potential and source temperature influenced the efficiency of H/D exchange. These suggest that the H/D exchange is likely to occur in the fore-vacuum region of the atmospheric interface. Analytical capabilities of the technique were demonstrated by differentiation of lysine and glutamine protonated molecular ions. Selective quantitation of lysine and glutamine mixtures was achieved, with a lower limit of detection of 1.5{\%} for glutamine and 0.2{\%} for lysine. H/D exchange of multiply charged macromolecular ions can also be carried out using this technique, which was demonstrated using cytochrome c.",
keywords = "Electrospray, H/D exchange, Ion-molecule reactions",
author = "Zolt{\'a}n Tak{\'a}ts and G. Schlosser and K. V{\'e}key",
year = "2003",
month = "8",
day = "15",
doi = "10.1016/S1387-3806(03)00234-3",
language = "English",
volume = "228",
pages = "729--741",
journal = "International Journal of Mass Spectrometry",
issn = "1387-3806",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Hydrogen/deuterium exchange of electrosprayed ions in the atmospheric interface of a commercial triple-quadrupole mass spectrometer

AU - Takáts, Zoltán

AU - Schlosser, G.

AU - Vékey, K.

PY - 2003/8/15

Y1 - 2003/8/15

N2 - A novel H/D exchange technique capable of the deuteration of electrosprayed ions has been developed. H/D exchange was carried out by introducing deuterating agent (e.g., d-MeOH) into the curtain gas flow of a commercial triple quadrupole mass spectrometer. In contrast to the widely used H/D exchange techniques, the ions are not trapped in this case. The main advantages of this technique are the ease of use and applicability on most commercial mass spectrometers, including quadrupole-type instruments. Effect of various instrumental parameters was investigated in detail, including spray voltage, spray position, partial pressure of the deuterating agent in the curtain gas, gas flow rates, the orifice-to-skimmer potential and the source temperature. Among these only the partial pressure of the deuterating agent in curtain gas, orifice-to-skimmer potential and source temperature influenced the efficiency of H/D exchange. These suggest that the H/D exchange is likely to occur in the fore-vacuum region of the atmospheric interface. Analytical capabilities of the technique were demonstrated by differentiation of lysine and glutamine protonated molecular ions. Selective quantitation of lysine and glutamine mixtures was achieved, with a lower limit of detection of 1.5% for glutamine and 0.2% for lysine. H/D exchange of multiply charged macromolecular ions can also be carried out using this technique, which was demonstrated using cytochrome c.

AB - A novel H/D exchange technique capable of the deuteration of electrosprayed ions has been developed. H/D exchange was carried out by introducing deuterating agent (e.g., d-MeOH) into the curtain gas flow of a commercial triple quadrupole mass spectrometer. In contrast to the widely used H/D exchange techniques, the ions are not trapped in this case. The main advantages of this technique are the ease of use and applicability on most commercial mass spectrometers, including quadrupole-type instruments. Effect of various instrumental parameters was investigated in detail, including spray voltage, spray position, partial pressure of the deuterating agent in the curtain gas, gas flow rates, the orifice-to-skimmer potential and the source temperature. Among these only the partial pressure of the deuterating agent in curtain gas, orifice-to-skimmer potential and source temperature influenced the efficiency of H/D exchange. These suggest that the H/D exchange is likely to occur in the fore-vacuum region of the atmospheric interface. Analytical capabilities of the technique were demonstrated by differentiation of lysine and glutamine protonated molecular ions. Selective quantitation of lysine and glutamine mixtures was achieved, with a lower limit of detection of 1.5% for glutamine and 0.2% for lysine. H/D exchange of multiply charged macromolecular ions can also be carried out using this technique, which was demonstrated using cytochrome c.

KW - Electrospray

KW - H/D exchange

KW - Ion-molecule reactions

UR - http://www.scopus.com/inward/record.url?scp=0041705977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041705977&partnerID=8YFLogxK

U2 - 10.1016/S1387-3806(03)00234-3

DO - 10.1016/S1387-3806(03)00234-3

M3 - Article

AN - SCOPUS:0041705977

VL - 228

SP - 729

EP - 741

JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

SN - 1387-3806

IS - 2-3

ER -