Hydrogen peroxide as a mediator of dopac-induced effects on melanoma cells

Eszter Karg, Evald Rosengren, Hans Rorsman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Dopac increases tyrosinase activity and exerts cytotoxic effects in cultures of human melanoma cells. The possible role of hydrogen peroxide in these actions was examined. Catalase (100 μg/ml) completely reversed the cytotoxic action of 0.3 mM dopac and reduced its tyrosinase-stimulating effect by approximately one half. The results show that extracellular hydrogen peroxide is a mediator of both the tyrosinase-stimulating and cytotoxic actions of dopac. Analysis of the degradation products of melanin from dopac-treated melanoma cells after hydriodic acid (HI) hydrolysis revealed the presence of aminohydroxy-phenylacetic acid (AHPAc). This substance is obtained by HI hydrolysis of melanin formed by oxidation of cysteinyl-dopac. Thus, the presence of AHPAc indicates that dopac is transported into the melanocytes where it serves as a substrate for tyrosinase.

Original languageEnglish
Pages (from-to)224-227
Number of pages4
JournalJournal of Investigative Dermatology
Volume96
Issue number2
Publication statusPublished - Feb 1991

Fingerprint

Monophenol Monooxygenase
Hydrogen Peroxide
Melanoma
Melanins
Hydrolysis
Melanocytes
Catalase
Degradation
Oxidation
Substrates
hydroiodic acid
phenylacetic acid

ASJC Scopus subject areas

  • Dermatology

Cite this

Hydrogen peroxide as a mediator of dopac-induced effects on melanoma cells. / Karg, Eszter; Rosengren, Evald; Rorsman, Hans.

In: Journal of Investigative Dermatology, Vol. 96, No. 2, 02.1991, p. 224-227.

Research output: Contribution to journalArticle

Karg, Eszter ; Rosengren, Evald ; Rorsman, Hans. / Hydrogen peroxide as a mediator of dopac-induced effects on melanoma cells. In: Journal of Investigative Dermatology. 1991 ; Vol. 96, No. 2. pp. 224-227.
@article{3477ba96578d42979fd1735f476e3b82,
title = "Hydrogen peroxide as a mediator of dopac-induced effects on melanoma cells",
abstract = "Dopac increases tyrosinase activity and exerts cytotoxic effects in cultures of human melanoma cells. The possible role of hydrogen peroxide in these actions was examined. Catalase (100 μg/ml) completely reversed the cytotoxic action of 0.3 mM dopac and reduced its tyrosinase-stimulating effect by approximately one half. The results show that extracellular hydrogen peroxide is a mediator of both the tyrosinase-stimulating and cytotoxic actions of dopac. Analysis of the degradation products of melanin from dopac-treated melanoma cells after hydriodic acid (HI) hydrolysis revealed the presence of aminohydroxy-phenylacetic acid (AHPAc). This substance is obtained by HI hydrolysis of melanin formed by oxidation of cysteinyl-dopac. Thus, the presence of AHPAc indicates that dopac is transported into the melanocytes where it serves as a substrate for tyrosinase.",
author = "Eszter Karg and Evald Rosengren and Hans Rorsman",
year = "1991",
month = "2",
language = "English",
volume = "96",
pages = "224--227",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Hydrogen peroxide as a mediator of dopac-induced effects on melanoma cells

AU - Karg, Eszter

AU - Rosengren, Evald

AU - Rorsman, Hans

PY - 1991/2

Y1 - 1991/2

N2 - Dopac increases tyrosinase activity and exerts cytotoxic effects in cultures of human melanoma cells. The possible role of hydrogen peroxide in these actions was examined. Catalase (100 μg/ml) completely reversed the cytotoxic action of 0.3 mM dopac and reduced its tyrosinase-stimulating effect by approximately one half. The results show that extracellular hydrogen peroxide is a mediator of both the tyrosinase-stimulating and cytotoxic actions of dopac. Analysis of the degradation products of melanin from dopac-treated melanoma cells after hydriodic acid (HI) hydrolysis revealed the presence of aminohydroxy-phenylacetic acid (AHPAc). This substance is obtained by HI hydrolysis of melanin formed by oxidation of cysteinyl-dopac. Thus, the presence of AHPAc indicates that dopac is transported into the melanocytes where it serves as a substrate for tyrosinase.

AB - Dopac increases tyrosinase activity and exerts cytotoxic effects in cultures of human melanoma cells. The possible role of hydrogen peroxide in these actions was examined. Catalase (100 μg/ml) completely reversed the cytotoxic action of 0.3 mM dopac and reduced its tyrosinase-stimulating effect by approximately one half. The results show that extracellular hydrogen peroxide is a mediator of both the tyrosinase-stimulating and cytotoxic actions of dopac. Analysis of the degradation products of melanin from dopac-treated melanoma cells after hydriodic acid (HI) hydrolysis revealed the presence of aminohydroxy-phenylacetic acid (AHPAc). This substance is obtained by HI hydrolysis of melanin formed by oxidation of cysteinyl-dopac. Thus, the presence of AHPAc indicates that dopac is transported into the melanocytes where it serves as a substrate for tyrosinase.

UR - http://www.scopus.com/inward/record.url?scp=0026072004&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026072004&partnerID=8YFLogxK

M3 - Article

C2 - 1899444

AN - SCOPUS:0026072004

VL - 96

SP - 224

EP - 227

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -