Hydrogen ion dependent reversible fibrillarization and hydration of chromatin in divalent cation-depleted isolated rat liver nuclei.

A. Miseta, A. Ludany, M. Kellermayer, K. Trombitas, C. F. Hazlewood

Research output: Contribution to journalArticle

Abstract

Rat liver cell nuclei isolated in the presence of Ca++ and Mg++ ions were exposed to citrate buffer at different pH levels. Their morphology was studied with the electron microscope and the water content measured. At a low pH the chromatin remained compact, but at neutral pH an intense fibrillarization of the chromatin was observed; and, seemed to be reversible, because of deoxyribonucleoproteins (DNP) filaments which dispersed at a pH of 7 were repacked to their previous site at a pH of 3. The ribonucleo-proteins (RNP)-containing structures did not show the same reversible fibrillarization. In addition to the morphological changes, a large increase in the water content ("water-holding capacity") of the chromatin structures was observed.

Original languageEnglish
Pages (from-to)3-8
Number of pages6
JournalPhysiological Chemistry and Physics and Medical NMR
Volume19
Issue number1
Publication statusPublished - 1987

Fingerprint

Divalent Cations
Hydration
Liver
Chromatin
Protons
Rats
Water content
Deoxyribonucleoproteins
Water
Citric Acid
Buffers
Electron microscopes
Cells
Ions
Cell Nucleus
Electrons
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Physiology
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Hydrogen ion dependent reversible fibrillarization and hydration of chromatin in divalent cation-depleted isolated rat liver nuclei. / Miseta, A.; Ludany, A.; Kellermayer, M.; Trombitas, K.; Hazlewood, C. F.

In: Physiological Chemistry and Physics and Medical NMR, Vol. 19, No. 1, 1987, p. 3-8.

Research output: Contribution to journalArticle

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AU - Hazlewood, C. F.

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