Hydrocarbon uptake by Streptomyces

Samir S. Radwan, György Barabás, Nasser A. Sorkhoh, Sándor Damjanovich, István Szabó, János Szöllosi, János Matkó, András Penyige, Tadashi Hirano, István M. Szabó

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Abstract

Oligocarbophilic Streptomyces strains capable of hydrocarbon uptake and utilization were isolated from the polluted desert of Kuwait and used in this study. Transmission electron-microscopy of hyphae revealed that they become enriched with large less electron dense areas in the cytoplasm, when biomass samples were incubated with alkanes. The Streptomyces isolate could utilize n-hexadecane as sole carbon and energy source and their fatty acid content showed an increase in the fatty acids with chain length equivalent to those of the alkane substrates. Fluorescence measurements of diphenylhexatriene dissolved in the representative alkane, n-hexadecane, showed that the kinetics of hydrocarbon uptake are quite different in hydrocarbon-utilizer compared with non-utilizer Streptomyces strain. Microviscosity of the cellular membrane of the utilizer strain was also different from that of the non-utilizer control strain Streptomyces griseus after incubation in the presence of n-hexadecane. Very likely the hydrocarbon utilizer transported these compounds more efficiently across their membranes and accumulated them as inclusions in the cytoplasm. Copyright (C) 1998 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalFEMS microbiology letters
Volume169
Issue number1
DOIs
Publication statusPublished - Dec 1 1998

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Keywords

  • Fluorescence anisotropy
  • Hydrocarbon utilization
  • Membrane viscosity
  • Streptomyces

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Radwan, S. S., Barabás, G., Sorkhoh, N. A., Damjanovich, S., Szabó, I., Szöllosi, J., Matkó, J., Penyige, A., Hirano, T., & Szabó, I. M. (1998). Hydrocarbon uptake by Streptomyces. FEMS microbiology letters, 169(1), 87-94. https://doi.org/10.1016/S0378-1097(98)00468-6