Hydrocarbon uptake by Streptomyces

Samir S. Radwan, György Barabás, Nasser A. Sorkhoh, S. Damjanovich, István Szabó, J. Szöllősi, J. Matkó, András Penyige, Tadashi Hirano, István M. Szabó

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Oligocarbophilic Streptomyces strains capable of hydrocarbon uptake and utilization were isolated from the polluted desert of Kuwait and used in this study. Transmission electron-microscopy of hyphae revealed that they become enriched with large less electron dense areas in the cytoplasm, when biomass samples were incubated with alkanes. The Streptomyces isolate could utilize n-hexadecane as sole carbon and energy source and their fatty acid content showed an increase in the fatty acids with chain length equivalent to those of the alkane substrates. Fluorescence measurements of diphenylhexatriene dissolved in the representative alkane, n-hexadecane, showed that the kinetics of hydrocarbon uptake are quite different in hydrocarbon-utilizer compared with non-utilizer Streptomyces strain. Microviscosity of the cellular membrane of the utilizer strain was also different from that of the non-utilizer control strain Streptomyces griseus after incubation in the presence of n-hexadecane. Very likely the hydrocarbon utilizer transported these compounds more efficiently across their membranes and accumulated them as inclusions in the cytoplasm. Copyright (C) 1998 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalFEMS Microbiology Letters
Volume169
Issue number1
DOIs
Publication statusPublished - Dec 1 1998

Fingerprint

Streptomyces
Hydrocarbons
Alkanes
Cytoplasm
Fatty Acids
Streptomyces griseus
Diphenylhexatriene
Kuwait
Membranes
Hyphae
Transmission Electron Microscopy
Biomass
Carbon
Fluorescence
Electrons
n-hexadecane

Keywords

  • Fluorescence anisotropy
  • Hydrocarbon utilization
  • Membrane viscosity
  • Streptomyces

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Radwan, S. S., Barabás, G., Sorkhoh, N. A., Damjanovich, S., Szabó, I., Szöllősi, J., ... Szabó, I. M. (1998). Hydrocarbon uptake by Streptomyces. FEMS Microbiology Letters, 169(1), 87-94. https://doi.org/10.1016/S0378-1097(98)00468-6

Hydrocarbon uptake by Streptomyces. / Radwan, Samir S.; Barabás, György; Sorkhoh, Nasser A.; Damjanovich, S.; Szabó, István; Szöllősi, J.; Matkó, J.; Penyige, András; Hirano, Tadashi; Szabó, István M.

In: FEMS Microbiology Letters, Vol. 169, No. 1, 01.12.1998, p. 87-94.

Research output: Contribution to journalArticle

Radwan, SS, Barabás, G, Sorkhoh, NA, Damjanovich, S, Szabó, I, Szöllősi, J, Matkó, J, Penyige, A, Hirano, T & Szabó, IM 1998, 'Hydrocarbon uptake by Streptomyces', FEMS Microbiology Letters, vol. 169, no. 1, pp. 87-94. https://doi.org/10.1016/S0378-1097(98)00468-6
Radwan, Samir S. ; Barabás, György ; Sorkhoh, Nasser A. ; Damjanovich, S. ; Szabó, István ; Szöllősi, J. ; Matkó, J. ; Penyige, András ; Hirano, Tadashi ; Szabó, István M. / Hydrocarbon uptake by Streptomyces. In: FEMS Microbiology Letters. 1998 ; Vol. 169, No. 1. pp. 87-94.
@article{15f0563a03174d7f94ef089118da7723,
title = "Hydrocarbon uptake by Streptomyces",
abstract = "Oligocarbophilic Streptomyces strains capable of hydrocarbon uptake and utilization were isolated from the polluted desert of Kuwait and used in this study. Transmission electron-microscopy of hyphae revealed that they become enriched with large less electron dense areas in the cytoplasm, when biomass samples were incubated with alkanes. The Streptomyces isolate could utilize n-hexadecane as sole carbon and energy source and their fatty acid content showed an increase in the fatty acids with chain length equivalent to those of the alkane substrates. Fluorescence measurements of diphenylhexatriene dissolved in the representative alkane, n-hexadecane, showed that the kinetics of hydrocarbon uptake are quite different in hydrocarbon-utilizer compared with non-utilizer Streptomyces strain. Microviscosity of the cellular membrane of the utilizer strain was also different from that of the non-utilizer control strain Streptomyces griseus after incubation in the presence of n-hexadecane. Very likely the hydrocarbon utilizer transported these compounds more efficiently across their membranes and accumulated them as inclusions in the cytoplasm. Copyright (C) 1998 Federation of European Microbiological Societies.",
keywords = "Fluorescence anisotropy, Hydrocarbon utilization, Membrane viscosity, Streptomyces",
author = "Radwan, {Samir S.} and Gy{\"o}rgy Barab{\'a}s and Sorkhoh, {Nasser A.} and S. Damjanovich and Istv{\'a}n Szab{\'o} and J. Sz{\"o}llősi and J. Matk{\'o} and Andr{\'a}s Penyige and Tadashi Hirano and Szab{\'o}, {Istv{\'a}n M.}",
year = "1998",
month = "12",
day = "1",
doi = "10.1016/S0378-1097(98)00468-6",
language = "English",
volume = "169",
pages = "87--94",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Hydrocarbon uptake by Streptomyces

AU - Radwan, Samir S.

AU - Barabás, György

AU - Sorkhoh, Nasser A.

AU - Damjanovich, S.

AU - Szabó, István

AU - Szöllősi, J.

AU - Matkó, J.

AU - Penyige, András

AU - Hirano, Tadashi

AU - Szabó, István M.

PY - 1998/12/1

Y1 - 1998/12/1

N2 - Oligocarbophilic Streptomyces strains capable of hydrocarbon uptake and utilization were isolated from the polluted desert of Kuwait and used in this study. Transmission electron-microscopy of hyphae revealed that they become enriched with large less electron dense areas in the cytoplasm, when biomass samples were incubated with alkanes. The Streptomyces isolate could utilize n-hexadecane as sole carbon and energy source and their fatty acid content showed an increase in the fatty acids with chain length equivalent to those of the alkane substrates. Fluorescence measurements of diphenylhexatriene dissolved in the representative alkane, n-hexadecane, showed that the kinetics of hydrocarbon uptake are quite different in hydrocarbon-utilizer compared with non-utilizer Streptomyces strain. Microviscosity of the cellular membrane of the utilizer strain was also different from that of the non-utilizer control strain Streptomyces griseus after incubation in the presence of n-hexadecane. Very likely the hydrocarbon utilizer transported these compounds more efficiently across their membranes and accumulated them as inclusions in the cytoplasm. Copyright (C) 1998 Federation of European Microbiological Societies.

AB - Oligocarbophilic Streptomyces strains capable of hydrocarbon uptake and utilization were isolated from the polluted desert of Kuwait and used in this study. Transmission electron-microscopy of hyphae revealed that they become enriched with large less electron dense areas in the cytoplasm, when biomass samples were incubated with alkanes. The Streptomyces isolate could utilize n-hexadecane as sole carbon and energy source and their fatty acid content showed an increase in the fatty acids with chain length equivalent to those of the alkane substrates. Fluorescence measurements of diphenylhexatriene dissolved in the representative alkane, n-hexadecane, showed that the kinetics of hydrocarbon uptake are quite different in hydrocarbon-utilizer compared with non-utilizer Streptomyces strain. Microviscosity of the cellular membrane of the utilizer strain was also different from that of the non-utilizer control strain Streptomyces griseus after incubation in the presence of n-hexadecane. Very likely the hydrocarbon utilizer transported these compounds more efficiently across their membranes and accumulated them as inclusions in the cytoplasm. Copyright (C) 1998 Federation of European Microbiological Societies.

KW - Fluorescence anisotropy

KW - Hydrocarbon utilization

KW - Membrane viscosity

KW - Streptomyces

UR - http://www.scopus.com/inward/record.url?scp=0031763323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031763323&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(98)00468-6

DO - 10.1016/S0378-1097(98)00468-6

M3 - Article

C2 - 9851038

AN - SCOPUS:0031763323

VL - 169

SP - 87

EP - 94

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -