Hybridization-modulated ion fluxes through peptide-nucleic-acid- functionalized gold nanotubes. A new approach to quantitative label-free DNA analysis

Gyula Jágerszki, R. Gyurcsányi, Lajos Höfler, Ernö Pretsch

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The inner walls of gold nanotubes, prepared by template synthesis in the nanopores of polycarbonate track etch membranes, have been chemically modified with peptide nucleic acid (PNA) and used for label-free quantification of complementary DNA sequences. Selective binding of DNA to the PNA-modified nanotubes is shown to decrease the flux of optically detected anionic markers through the nanotubes in a concentration-dependent manner. The strong dependence of the biorecognition-modulated ion transport through the nanopores on the ionic strength suggests a dominantly electrostatic exclusion mechanism of the ion flux decrease as a result of DNA binding to the PNA-modified nanopores.

Original languageEnglish
Pages (from-to)1609-1612
Number of pages4
JournalNano Letters
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Peptide Nucleic Acids
Nanopores
nucleic acids
Gold
Nanotubes
peptides
Labels
nanotubes
polycarbonate
DNA
deoxyribonucleic acid
Ions
gold
Fluxes
complementary DNA
ions
DNA sequences
polycarbonates
Polycarbonates
Ionic strength

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

Cite this

Hybridization-modulated ion fluxes through peptide-nucleic-acid- functionalized gold nanotubes. A new approach to quantitative label-free DNA analysis. / Jágerszki, Gyula; Gyurcsányi, R.; Höfler, Lajos; Pretsch, Ernö.

In: Nano Letters, Vol. 7, No. 6, 06.2007, p. 1609-1612.

Research output: Contribution to journalArticle

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