A hibridizáció és alkalmazása DNS-array rendszerekben

Translated title of the contribution: Hybridization and their application in the DNA array technology

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Abstract

DNA hybridization arrays measure simultaneously the expression of several genes. First, a known DNA sequence (probe) is fixed on a firm basis. Then the complementer sequence (target sequence) is linked to it during the hybridization process. The target sequence extracted from biological samples is fluorescently, enzimatically or radioactivelly labeled before detection. Higher expression results in higher signal in the detection system. Unlabeled DNA strands can also be detected, as the electronical and optical characteristics of the DNA is altered after complementer hybridization. In this review we summarize the basics of hybridisation and its newest application area in the DNA array systems.

Original languageHungarian
Pages (from-to)1447-1452
Number of pages6
JournalOrvosi hetilap
Volume146
Issue number27
Publication statusPublished - Dec 1 2005

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ASJC Scopus subject areas

  • Medicine(all)

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