Hybrid intracerebral probe with integrated bare LED chips for optogenetic studies

Suleman Ayub, Luc J. Gentet, Richárd Fiáth, Michael Schwaerzle, Mélodie Borel, François David, P. Barthó, I. Ulbert, Oliver Paul, Patrick Ruther

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This article reports on the development, i.e., the design, fabrication, and validation of an implantable optical neural probes designed for in vivo experiments relying on optogenetics. The probes comprise an array of ten bare light-emitting diode (LED) chips emitting at a wavelength of 460 nm and integrated along a flexible polyimide-based substrate stiffened using a micromachined ladder-like silicon structure. The resulting mechanical stiffness of the slender, 250-μm-wide, 65-μm-thick, and 5- and 8-mm-long probe shank facilitates its implantation into neural tissue. The LEDs are encapsulated by a fluropolymer coating protecting the implant against the physiological conditions in the brain. The electrical interface to the external control unit is provided by 10-μm-thick, highly flexible polyimide cables making the probes suitable for both acute and chronic in vivo experiments. Optical and electrical properties of the probes are reported, as well as their in vivo validation in acute optogenetic studies in transgenic mice. The depth-dependent optical stimulation of both excitatory and inhibitory neurons is demonstrated by altering the brain activity in the cortex and the thalamus. Local network responses elicited by 20-ms-long light pulses of different optical power (20 μW and 1 mW), as well as local modulation of single unit neuronal activity to 1-s-long light pulses with low optical intensity (17 μW) are presented. The ability to modulate neural activity makes these devices suitable for a broad variety of optogenetic experiments.

Original languageEnglish
Article number49
JournalBiomedical Microdevices
Volume19
Issue number3
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

Optogenetics
Light emitting diodes
Light
Polyimides
Brain
Silicon
Thalamus
Transgenic Mice
Experiments
Ladders
Neurons
Equipment and Supplies
Cables
Electric properties
Optical properties
Stiffness
Modulation
Tissue
Fabrication
Coatings

Keywords

  • In vivo application
  • Light-emitting diodes (LEDs)
  • Neural probes
  • Optical probe
  • Optogenetics

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

Cite this

Ayub, S., Gentet, L. J., Fiáth, R., Schwaerzle, M., Borel, M., David, F., ... Ruther, P. (2017). Hybrid intracerebral probe with integrated bare LED chips for optogenetic studies. Biomedical Microdevices, 19(3), [49]. https://doi.org/10.1007/s10544-017-0190-3

Hybrid intracerebral probe with integrated bare LED chips for optogenetic studies. / Ayub, Suleman; Gentet, Luc J.; Fiáth, Richárd; Schwaerzle, Michael; Borel, Mélodie; David, François; Barthó, P.; Ulbert, I.; Paul, Oliver; Ruther, Patrick.

In: Biomedical Microdevices, Vol. 19, No. 3, 49, 01.09.2017.

Research output: Contribution to journalArticle

Ayub, S, Gentet, LJ, Fiáth, R, Schwaerzle, M, Borel, M, David, F, Barthó, P, Ulbert, I, Paul, O & Ruther, P 2017, 'Hybrid intracerebral probe with integrated bare LED chips for optogenetic studies', Biomedical Microdevices, vol. 19, no. 3, 49. https://doi.org/10.1007/s10544-017-0190-3
Ayub, Suleman ; Gentet, Luc J. ; Fiáth, Richárd ; Schwaerzle, Michael ; Borel, Mélodie ; David, François ; Barthó, P. ; Ulbert, I. ; Paul, Oliver ; Ruther, Patrick. / Hybrid intracerebral probe with integrated bare LED chips for optogenetic studies. In: Biomedical Microdevices. 2017 ; Vol. 19, No. 3.
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