Hybrid 5 S ribosomal RNA encoded by a multicopy plasmid is incorporated into ribosomes of Escherichia coli

J. Szeberényi, David Apirion

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The recombinant plasmid pJR3Δ contains a tandem pair of promoters from rrnA followed by a hybrid 5 S rRNA gene, derived from the two 5 S rRNA genes of the rrnD transcription unit, and a terminator. Escherichia coli cells transformed with this plasmid produce 2-3-times more 5 S rRNA compared to untransformed cells. The growth of cells containing this plasmid is not affected significantly. Although the sequence and the secondary structure of the plasmid-specific 5 S rRNA differ from those of its counterparts (e.g., from 5 S rRNA species encoded by chromosomal genes), it is processed properly and is incorporated into ribosomes.

Original languageEnglish
Pages (from-to)17-20
Number of pages4
JournalFEBS Letters
Volume169
Issue number1
DOIs
Publication statusPublished - Apr 9 1984

Fingerprint

Ribosomal RNA
Ribosomes
Escherichia coli
Plasmids
Genes
rRNA Genes
Transcription
Cells
Growth

Keywords

  • Fingerpriting
  • Hybrid 5 S rRNA
  • Molecular stalk
  • Recombinant plasmid
  • Ribosome
  • RNA processing

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hybrid 5 S ribosomal RNA encoded by a multicopy plasmid is incorporated into ribosomes of Escherichia coli. / Szeberényi, J.; Apirion, David.

In: FEBS Letters, Vol. 169, No. 1, 09.04.1984, p. 17-20.

Research output: Contribution to journalArticle

@article{095a1766b5e645df949839991dde4122,
title = "Hybrid 5 S ribosomal RNA encoded by a multicopy plasmid is incorporated into ribosomes of Escherichia coli",
abstract = "The recombinant plasmid pJR3Δ contains a tandem pair of promoters from rrnA followed by a hybrid 5 S rRNA gene, derived from the two 5 S rRNA genes of the rrnD transcription unit, and a terminator. Escherichia coli cells transformed with this plasmid produce 2-3-times more 5 S rRNA compared to untransformed cells. The growth of cells containing this plasmid is not affected significantly. Although the sequence and the secondary structure of the plasmid-specific 5 S rRNA differ from those of its counterparts (e.g., from 5 S rRNA species encoded by chromosomal genes), it is processed properly and is incorporated into ribosomes.",
keywords = "Fingerpriting, Hybrid 5 S rRNA, Molecular stalk, Recombinant plasmid, Ribosome, RNA processing",
author = "J. Szeber{\'e}nyi and David Apirion",
year = "1984",
month = "4",
day = "9",
doi = "10.1016/0014-5793(84)80280-X",
language = "English",
volume = "169",
pages = "17--20",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Hybrid 5 S ribosomal RNA encoded by a multicopy plasmid is incorporated into ribosomes of Escherichia coli

AU - Szeberényi, J.

AU - Apirion, David

PY - 1984/4/9

Y1 - 1984/4/9

N2 - The recombinant plasmid pJR3Δ contains a tandem pair of promoters from rrnA followed by a hybrid 5 S rRNA gene, derived from the two 5 S rRNA genes of the rrnD transcription unit, and a terminator. Escherichia coli cells transformed with this plasmid produce 2-3-times more 5 S rRNA compared to untransformed cells. The growth of cells containing this plasmid is not affected significantly. Although the sequence and the secondary structure of the plasmid-specific 5 S rRNA differ from those of its counterparts (e.g., from 5 S rRNA species encoded by chromosomal genes), it is processed properly and is incorporated into ribosomes.

AB - The recombinant plasmid pJR3Δ contains a tandem pair of promoters from rrnA followed by a hybrid 5 S rRNA gene, derived from the two 5 S rRNA genes of the rrnD transcription unit, and a terminator. Escherichia coli cells transformed with this plasmid produce 2-3-times more 5 S rRNA compared to untransformed cells. The growth of cells containing this plasmid is not affected significantly. Although the sequence and the secondary structure of the plasmid-specific 5 S rRNA differ from those of its counterparts (e.g., from 5 S rRNA species encoded by chromosomal genes), it is processed properly and is incorporated into ribosomes.

KW - Fingerpriting

KW - Hybrid 5 S rRNA

KW - Molecular stalk

KW - Recombinant plasmid

KW - Ribosome

KW - RNA processing

UR - http://www.scopus.com/inward/record.url?scp=0021758687&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021758687&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(84)80280-X

DO - 10.1016/0014-5793(84)80280-X

M3 - Article

VL - 169

SP - 17

EP - 20

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -