HvPG1 and ECA1: Two genes activated transcriptionally in the transition of barley microspores from the gametophytic to the embryogenic pathway

Amada Pulido, Ferenc Bakos, Martine Devic, Beáta Barnabás, Adela Olmedilla

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Microspores genetically programmed to produce male gametes can be switched to the embryogenic pathway to give rise to haploid embryos. Microspore embryogenesis is usually induced in barley by stress pre-treatment applied to vacuolated microspores. We studied the expression of two genes during the early stages of microspore embryogenesis to gain further insight into the microspore transition from the gametophytic to the embryogenic pathway. RT-PCR together with in situ hybridization on sections (ISH) and whole-mount in situ hybridization (WISH) were used to analyse the expression of the early-culture abundant gene (ECA1), which is expressed in barley during microspore embryogenesis, and a polygalacturonase gene (HvPG1), a late pollen gene expressed during gametogenesis only after microspore division. Both ECA1 and HvPG1 genes were transcriptionally active after stress pre-treatment in the same populations of microspore-derived structures, representing the sporophytically induced ones. ECA1 transcripts were also detected after 3 days' culture. Our results point to the possibility of using ECA1 gene expression as a marker for the induction of microspore embryogenesis and the earliest stages of this process. Finally, we demonstrate that WISH is a suitable technique for studying gene expression in embryogenic microspore populations and, because different structures can be examined individually, is an appropriate complement to transcriptomic profile analyses in the study of early microspore embryogenesis.

Original languageEnglish
Pages (from-to)551-559
Number of pages9
JournalPlant Cell Reports
Volume28
Issue number4
DOIs
Publication statusPublished - Apr 1 2009

Keywords

  • Barley
  • Gametophytic development
  • Microspore embryogenesis
  • Molecular marker
  • Whole-mount in situ hybridization

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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