Humoral immune response to cytomegalovirus Towne vaccine strain and to Toledo low-passage strain

E. Gönczöl, J. Ianacone, G. Furlini, W. Ho, S. A. Plotkin

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Neutralization and immunoblot or immunoprecipitation assays of serum samples from seronegative or seropositive volunteers immunized with the attenuated Towne and challenged with the virulent Toledo cytomegalovirus strains were carried out. Titers of neutralizing antibodies differed as a function of the strain used for immunization. All serum samples with neutralizing activity detected a 58-kDa protein that is the abundant component of the major glycoprotein complex of the envelope, suggesting that this protein complex is involved in the induction of neutralizing antibodies. Complement-independent neutralizing activity was found to develop later than complement-dependent activity, and no correlation was observed between complement-independent titers of neutralizing antibodies and antibody to the 86-kDa protein, which bears a complement-independent neutralizing epitope. Antibodies to the 66-kDa major tegument protein were present early after infection but were not correlated with serum neutralizing activity.

Original languageEnglish
Pages (from-to)851-859
Number of pages9
JournalJournal of Infectious Diseases
Volume159
Issue number5
Publication statusPublished - 1989

Fingerprint

Cytomegalovirus Vaccines
Humoral Immunity
Neutralizing Antibodies
Proteins
Serum
Antibodies
Cytomegalovirus
Immunoprecipitation
Epitopes
Volunteers
Immunization
Glycoproteins
Infection

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Humoral immune response to cytomegalovirus Towne vaccine strain and to Toledo low-passage strain. / Gönczöl, E.; Ianacone, J.; Furlini, G.; Ho, W.; Plotkin, S. A.

In: Journal of Infectious Diseases, Vol. 159, No. 5, 1989, p. 851-859.

Research output: Contribution to journalArticle

Gönczöl, E. ; Ianacone, J. ; Furlini, G. ; Ho, W. ; Plotkin, S. A. / Humoral immune response to cytomegalovirus Towne vaccine strain and to Toledo low-passage strain. In: Journal of Infectious Diseases. 1989 ; Vol. 159, No. 5. pp. 851-859.
@article{fe73fe1c1e3f48b9a8caee734e1c3a40,
title = "Humoral immune response to cytomegalovirus Towne vaccine strain and to Toledo low-passage strain",
abstract = "Neutralization and immunoblot or immunoprecipitation assays of serum samples from seronegative or seropositive volunteers immunized with the attenuated Towne and challenged with the virulent Toledo cytomegalovirus strains were carried out. Titers of neutralizing antibodies differed as a function of the strain used for immunization. All serum samples with neutralizing activity detected a 58-kDa protein that is the abundant component of the major glycoprotein complex of the envelope, suggesting that this protein complex is involved in the induction of neutralizing antibodies. Complement-independent neutralizing activity was found to develop later than complement-dependent activity, and no correlation was observed between complement-independent titers of neutralizing antibodies and antibody to the 86-kDa protein, which bears a complement-independent neutralizing epitope. Antibodies to the 66-kDa major tegument protein were present early after infection but were not correlated with serum neutralizing activity.",
author = "E. G{\"o}ncz{\"o}l and J. Ianacone and G. Furlini and W. Ho and Plotkin, {S. A.}",
year = "1989",
language = "English",
volume = "159",
pages = "851--859",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Humoral immune response to cytomegalovirus Towne vaccine strain and to Toledo low-passage strain

AU - Gönczöl, E.

AU - Ianacone, J.

AU - Furlini, G.

AU - Ho, W.

AU - Plotkin, S. A.

PY - 1989

Y1 - 1989

N2 - Neutralization and immunoblot or immunoprecipitation assays of serum samples from seronegative or seropositive volunteers immunized with the attenuated Towne and challenged with the virulent Toledo cytomegalovirus strains were carried out. Titers of neutralizing antibodies differed as a function of the strain used for immunization. All serum samples with neutralizing activity detected a 58-kDa protein that is the abundant component of the major glycoprotein complex of the envelope, suggesting that this protein complex is involved in the induction of neutralizing antibodies. Complement-independent neutralizing activity was found to develop later than complement-dependent activity, and no correlation was observed between complement-independent titers of neutralizing antibodies and antibody to the 86-kDa protein, which bears a complement-independent neutralizing epitope. Antibodies to the 66-kDa major tegument protein were present early after infection but were not correlated with serum neutralizing activity.

AB - Neutralization and immunoblot or immunoprecipitation assays of serum samples from seronegative or seropositive volunteers immunized with the attenuated Towne and challenged with the virulent Toledo cytomegalovirus strains were carried out. Titers of neutralizing antibodies differed as a function of the strain used for immunization. All serum samples with neutralizing activity detected a 58-kDa protein that is the abundant component of the major glycoprotein complex of the envelope, suggesting that this protein complex is involved in the induction of neutralizing antibodies. Complement-independent neutralizing activity was found to develop later than complement-dependent activity, and no correlation was observed between complement-independent titers of neutralizing antibodies and antibody to the 86-kDa protein, which bears a complement-independent neutralizing epitope. Antibodies to the 66-kDa major tegument protein were present early after infection but were not correlated with serum neutralizing activity.

UR - http://www.scopus.com/inward/record.url?scp=0024566561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024566561&partnerID=8YFLogxK

M3 - Article

C2 - 2540246

AN - SCOPUS:0024566561

VL - 159

SP - 851

EP - 859

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -