Human stem cell factor (c-kit ligand) induces an autocrine loop of growth in a GM-CSF-dependent megakaryocytic leukemia cell line

C. Kiss, Alessandra Cesano, Krisztina M. Zsebö, Steven C. Clark, Daniela Santoli

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The M07e megakaryoblastic leukemia cell line is strictly dependent on either interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for continuous growth. This study shows that recombinant human stem-cell factor (rhSCF) can completely replace these lymphokines in supporting the continued propagation of M07e cells mostly by eliciting GM-CSF secretion in this target. In fact, in short-term proliferation assays the stimulatory activity of SCF is blocked about 75% by a GM-CSF-specific serum. In addition, we could detect GM-CSF expression by SCF-stimulated M07e cells, both at the protein and mRNA levels. In contrast, SCF does not induce transcripts for any other cytokine to which M07e cells are responsive, including IL-2, IL-3, IL-4, and IL-6. Overall, these data show that the ability of SCF to support the growth of this megakaryocytic cell line is mediated mostly by the induction of an autocrine loop of activation involving GM-CSF production. The finding that SCF can stimulate GM-CSF secretion also in an IL-2-dependent T-lymphoblastic leukemia cell line indicates that SCF can act on cells of both myeloid and lymphoid lineages, and that the ability to induce cytokines in target cells represents an important aspect of its mechanism of action.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalLeukemia
Volume7
Issue number2
Publication statusPublished - Feb 1993

Fingerprint

Leukemia, Megakaryoblastic, Acute
Stem Cell Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Cell Line
Growth
Interleukin-3
Interleukin-2
Cytokines
Lymphokines
Myeloid Cells
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Interleukin-4
Interleukin-6
Leukemia
Lymphocytes
Messenger RNA
Serum

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Human stem cell factor (c-kit ligand) induces an autocrine loop of growth in a GM-CSF-dependent megakaryocytic leukemia cell line. / Kiss, C.; Cesano, Alessandra; Zsebö, Krisztina M.; Clark, Steven C.; Santoli, Daniela.

In: Leukemia, Vol. 7, No. 2, 02.1993, p. 235-240.

Research output: Contribution to journalArticle

Kiss, C. ; Cesano, Alessandra ; Zsebö, Krisztina M. ; Clark, Steven C. ; Santoli, Daniela. / Human stem cell factor (c-kit ligand) induces an autocrine loop of growth in a GM-CSF-dependent megakaryocytic leukemia cell line. In: Leukemia. 1993 ; Vol. 7, No. 2. pp. 235-240.
@article{8fef56fc79744902940f0dca75410e40,
title = "Human stem cell factor (c-kit ligand) induces an autocrine loop of growth in a GM-CSF-dependent megakaryocytic leukemia cell line",
abstract = "The M07e megakaryoblastic leukemia cell line is strictly dependent on either interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for continuous growth. This study shows that recombinant human stem-cell factor (rhSCF) can completely replace these lymphokines in supporting the continued propagation of M07e cells mostly by eliciting GM-CSF secretion in this target. In fact, in short-term proliferation assays the stimulatory activity of SCF is blocked about 75{\%} by a GM-CSF-specific serum. In addition, we could detect GM-CSF expression by SCF-stimulated M07e cells, both at the protein and mRNA levels. In contrast, SCF does not induce transcripts for any other cytokine to which M07e cells are responsive, including IL-2, IL-3, IL-4, and IL-6. Overall, these data show that the ability of SCF to support the growth of this megakaryocytic cell line is mediated mostly by the induction of an autocrine loop of activation involving GM-CSF production. The finding that SCF can stimulate GM-CSF secretion also in an IL-2-dependent T-lymphoblastic leukemia cell line indicates that SCF can act on cells of both myeloid and lymphoid lineages, and that the ability to induce cytokines in target cells represents an important aspect of its mechanism of action.",
author = "C. Kiss and Alessandra Cesano and Zseb{\"o}, {Krisztina M.} and Clark, {Steven C.} and Daniela Santoli",
year = "1993",
month = "2",
language = "English",
volume = "7",
pages = "235--240",
journal = "Leukemia",
issn = "0887-6924",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Human stem cell factor (c-kit ligand) induces an autocrine loop of growth in a GM-CSF-dependent megakaryocytic leukemia cell line

AU - Kiss, C.

AU - Cesano, Alessandra

AU - Zsebö, Krisztina M.

AU - Clark, Steven C.

AU - Santoli, Daniela

PY - 1993/2

Y1 - 1993/2

N2 - The M07e megakaryoblastic leukemia cell line is strictly dependent on either interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for continuous growth. This study shows that recombinant human stem-cell factor (rhSCF) can completely replace these lymphokines in supporting the continued propagation of M07e cells mostly by eliciting GM-CSF secretion in this target. In fact, in short-term proliferation assays the stimulatory activity of SCF is blocked about 75% by a GM-CSF-specific serum. In addition, we could detect GM-CSF expression by SCF-stimulated M07e cells, both at the protein and mRNA levels. In contrast, SCF does not induce transcripts for any other cytokine to which M07e cells are responsive, including IL-2, IL-3, IL-4, and IL-6. Overall, these data show that the ability of SCF to support the growth of this megakaryocytic cell line is mediated mostly by the induction of an autocrine loop of activation involving GM-CSF production. The finding that SCF can stimulate GM-CSF secretion also in an IL-2-dependent T-lymphoblastic leukemia cell line indicates that SCF can act on cells of both myeloid and lymphoid lineages, and that the ability to induce cytokines in target cells represents an important aspect of its mechanism of action.

AB - The M07e megakaryoblastic leukemia cell line is strictly dependent on either interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for continuous growth. This study shows that recombinant human stem-cell factor (rhSCF) can completely replace these lymphokines in supporting the continued propagation of M07e cells mostly by eliciting GM-CSF secretion in this target. In fact, in short-term proliferation assays the stimulatory activity of SCF is blocked about 75% by a GM-CSF-specific serum. In addition, we could detect GM-CSF expression by SCF-stimulated M07e cells, both at the protein and mRNA levels. In contrast, SCF does not induce transcripts for any other cytokine to which M07e cells are responsive, including IL-2, IL-3, IL-4, and IL-6. Overall, these data show that the ability of SCF to support the growth of this megakaryocytic cell line is mediated mostly by the induction of an autocrine loop of activation involving GM-CSF production. The finding that SCF can stimulate GM-CSF secretion also in an IL-2-dependent T-lymphoblastic leukemia cell line indicates that SCF can act on cells of both myeloid and lymphoid lineages, and that the ability to induce cytokines in target cells represents an important aspect of its mechanism of action.

UR - http://www.scopus.com/inward/record.url?scp=0027515956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027515956&partnerID=8YFLogxK

M3 - Article

C2 - 7678880

AN - SCOPUS:0027515956

VL - 7

SP - 235

EP - 240

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 2

ER -