Human Stem-Cell-Derived Cardiomyocytes in Drug Discovery and Toxicity Testing

Kornélia Szebényi, Adrienn Péntek, Nóra Varga, Zsuzsa Erdei, Gergo Vofély, Tamás I. Orbán, Balázs Sarkadi, Ágota Apáti

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter, we review the methods of cardiomyocyte generation from human pluripotent and somatic stem cells, as well as from adult tissues through transdifferentiation. We describe the methods for stable genetic modification of human pluripotent stem cells, which can be applied to generate appropriate cardiomyocytes (CM) for drug screening, and introduce current methodologies for the preparation of cardiac progenitors for large-scale production. An overview of the current technologies for examining cardiotoxicity and the related key screening systems for drug development is provided. We describe selective reporter systems, which can be introduced into differentiated human CM and the potential application of human CM for disease models and personalized drug development. Finally, the future perspectives of these special systems in drug discovery are discussed.

Original languageEnglish
Title of host publicationHandbook of Nanotoxicology, Nanomedicine and Stem Cell Use in Toxicology
PublisherWiley Blackwell
Pages267-298
Number of pages32
ISBN (Electronic)9781118856017
ISBN (Print)9781118439265
DOIs
Publication statusPublished - Jun 3 2014

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Keywords

  • Cardiac differentiation
  • Cardiotoxicity
  • Disease model
  • Gene delivery
  • Human embryonic stem cell
  • Induced pluripotent stem cell
  • Personalized medicine
  • Reporter system

ASJC Scopus subject areas

  • Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Materials Science(all)

Cite this

Szebényi, K., Péntek, A., Varga, N., Erdei, Z., Vofély, G., Orbán, T. I., Sarkadi, B., & Apáti, Á. (2014). Human Stem-Cell-Derived Cardiomyocytes in Drug Discovery and Toxicity Testing. In Handbook of Nanotoxicology, Nanomedicine and Stem Cell Use in Toxicology (pp. 267-298). Wiley Blackwell. https://doi.org/10.1002/9781118856017.ch15