Human recombinant macrophage colony-stimulating factor (M-CSF) increases Cl-esterase inhibitor (Cl-INH) synthesis by human monocytes

B. Schmidt, G. Gyapay, M. Valay, G. Füst

Research output: Contribution to journalArticle

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Abstract

The ability of macrophage colony-stimulating factor (M-CSF) to influence production of complement proteins by cultured human monocytes was studied. Three-day-old cultures of human monocytes were treated with 250-1000 U/ml recombinant human M-CSF (Cetus Corporation, Emeryville, CA). After 3 days the M-CSF containing medium was replaced by the same medium without M-CSF, and the cells were cultured for 3 more days. Samples taken at the removal of M-CSF and 3 days later were tested for the concentration of factor B and Cl-esterase inhibitor (Cl-INH) using ELISA methods, and C2 by using an immunohaemolytic assay. M-CSF induced a marked dose-dependent increase in the synthesis of Cl-INH, but did not significantly change Bf and C2 production. The findings suggest that M-CSF is able to influence selectively the complement protein-producing ability of cultured human monocytes.

Original languageEnglish
Pages (from-to)677-679
Number of pages3
JournalImmunology
Volume74
Issue number4
Publication statusPublished - 1991

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Macrophage Colony-Stimulating Factor
Esterases
Monocytes
Complement System Proteins
Complement Factor B
Cultured Cells
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Immunology

Cite this

Human recombinant macrophage colony-stimulating factor (M-CSF) increases Cl-esterase inhibitor (Cl-INH) synthesis by human monocytes. / Schmidt, B.; Gyapay, G.; Valay, M.; Füst, G.

In: Immunology, Vol. 74, No. 4, 1991, p. 677-679.

Research output: Contribution to journalArticle

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