A rekombináns humán erythropoietin-α fokozza a humán laphámrák sugárérzékenysé gét preklinikai modellben

Translated title of the contribution: Human recombinant erythropoietin-a increases the efficacy of irradiation in preclinical model

József Lövey, I. Kenessey, E. Rásó, J. Dobos, Ágnes Vágó, M. Kásler, Krisztina Futosi, B. Döme, J. Tímár, J. Tóvári

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

According to recent data erythropoietin receptor (EPOR) is expressed not only by bone marrow erythroid progenitors but by endothelial- and cancer cells and it was suggested that erythropoietin (EPO) may affect their functions as well. We have analyzed the effects of recombinant human erythropoietin-a ?(rHuEPOa) on radiation sensitivity of EPOR+ human epidermoid carcinoma (A431) xenograft model. In vivo rHuEPOa treatment was started after tumor cell inoculation into SCID mice. 5 Gy irradiation was performed on day 14, the effect of which was measured on day 22. Hemoglobin level, tumor-associated microvessels and HIF-1α expression of the xenograft were monitored during the experiment. rHuEPOa administration prevented the development of tumorinduced anemia of SCID mice and reduced the level of HIF-1α expression of the xenograft tumor without affecting tumor growth. We have found that rHuEPOa treatment significantly increased the efficacy of antitumor effect of irradiation which was partly mediated by complex effects on tumorassociated microvessels. In vitro rHuEPOa did not affect proliferation of A431 cells but enhanced the antiproliferative and proapoptotic effects of irradiation. We concluded that rHuEPOa administration positively modulated the antitumoral effects of irradiation at least by two pathways, decreasing systemic hypoxia resulting in decreased tumoral HIF-1α expression and enhancing direct effects on tumor-associated microvessels.

Original languageHungarian
Pages (from-to)53-61
Number of pages9
JournalMagyar Onkologia
Volume51
Issue number1
Publication statusPublished - 2007

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Erythropoietin
Microvessels
Heterografts
Erythropoietin Receptors
Neoplasms
SCID Mice
Radiation Tolerance
Anemia
Squamous Cell Carcinoma
Hemoglobins
Bone Marrow
Cell Proliferation
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

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title = "A rekombin{\'a}ns hum{\'a}n erythropoietin-α fokozza a hum{\'a}n laph{\'a}mr{\'a}k sug{\'a}r{\'e}rz{\'e}kenys{\'e} g{\'e}t preklinikai modellben",
abstract = "According to recent data erythropoietin receptor (EPOR) is expressed not only by bone marrow erythroid progenitors but by endothelial- and cancer cells and it was suggested that erythropoietin (EPO) may affect their functions as well. We have analyzed the effects of recombinant human erythropoietin-a ?(rHuEPOa) on radiation sensitivity of EPOR+ human epidermoid carcinoma (A431) xenograft model. In vivo rHuEPOa treatment was started after tumor cell inoculation into SCID mice. 5 Gy irradiation was performed on day 14, the effect of which was measured on day 22. Hemoglobin level, tumor-associated microvessels and HIF-1α expression of the xenograft were monitored during the experiment. rHuEPOa administration prevented the development of tumorinduced anemia of SCID mice and reduced the level of HIF-1α expression of the xenograft tumor without affecting tumor growth. We have found that rHuEPOa treatment significantly increased the efficacy of antitumor effect of irradiation which was partly mediated by complex effects on tumorassociated microvessels. In vitro rHuEPOa did not affect proliferation of A431 cells but enhanced the antiproliferative and proapoptotic effects of irradiation. We concluded that rHuEPOa administration positively modulated the antitumoral effects of irradiation at least by two pathways, decreasing systemic hypoxia resulting in decreased tumoral HIF-1α expression and enhancing direct effects on tumor-associated microvessels.",
author = "J{\'o}zsef L{\"o}vey and I. Kenessey and E. R{\'a}s{\'o} and J. Dobos and {\'A}gnes V{\'a}g{\'o} and M. K{\'a}sler and Krisztina Futosi and B. D{\"o}me and J. T{\'i}m{\'a}r and J. T{\'o}v{\'a}ri",
year = "2007",
language = "Hungarian",
volume = "51",
pages = "53--61",
journal = "Magyar Onkologia",
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T1 - A rekombináns humán erythropoietin-α fokozza a humán laphámrák sugárérzékenysé gét preklinikai modellben

AU - Lövey, József

AU - Kenessey, I.

AU - Rásó, E.

AU - Dobos, J.

AU - Vágó, Ágnes

AU - Kásler, M.

AU - Futosi, Krisztina

AU - Döme, B.

AU - Tímár, J.

AU - Tóvári, J.

PY - 2007

Y1 - 2007

N2 - According to recent data erythropoietin receptor (EPOR) is expressed not only by bone marrow erythroid progenitors but by endothelial- and cancer cells and it was suggested that erythropoietin (EPO) may affect their functions as well. We have analyzed the effects of recombinant human erythropoietin-a ?(rHuEPOa) on radiation sensitivity of EPOR+ human epidermoid carcinoma (A431) xenograft model. In vivo rHuEPOa treatment was started after tumor cell inoculation into SCID mice. 5 Gy irradiation was performed on day 14, the effect of which was measured on day 22. Hemoglobin level, tumor-associated microvessels and HIF-1α expression of the xenograft were monitored during the experiment. rHuEPOa administration prevented the development of tumorinduced anemia of SCID mice and reduced the level of HIF-1α expression of the xenograft tumor without affecting tumor growth. We have found that rHuEPOa treatment significantly increased the efficacy of antitumor effect of irradiation which was partly mediated by complex effects on tumorassociated microvessels. In vitro rHuEPOa did not affect proliferation of A431 cells but enhanced the antiproliferative and proapoptotic effects of irradiation. We concluded that rHuEPOa administration positively modulated the antitumoral effects of irradiation at least by two pathways, decreasing systemic hypoxia resulting in decreased tumoral HIF-1α expression and enhancing direct effects on tumor-associated microvessels.

AB - According to recent data erythropoietin receptor (EPOR) is expressed not only by bone marrow erythroid progenitors but by endothelial- and cancer cells and it was suggested that erythropoietin (EPO) may affect their functions as well. We have analyzed the effects of recombinant human erythropoietin-a ?(rHuEPOa) on radiation sensitivity of EPOR+ human epidermoid carcinoma (A431) xenograft model. In vivo rHuEPOa treatment was started after tumor cell inoculation into SCID mice. 5 Gy irradiation was performed on day 14, the effect of which was measured on day 22. Hemoglobin level, tumor-associated microvessels and HIF-1α expression of the xenograft were monitored during the experiment. rHuEPOa administration prevented the development of tumorinduced anemia of SCID mice and reduced the level of HIF-1α expression of the xenograft tumor without affecting tumor growth. We have found that rHuEPOa treatment significantly increased the efficacy of antitumor effect of irradiation which was partly mediated by complex effects on tumorassociated microvessels. In vitro rHuEPOa did not affect proliferation of A431 cells but enhanced the antiproliferative and proapoptotic effects of irradiation. We concluded that rHuEPOa administration positively modulated the antitumoral effects of irradiation at least by two pathways, decreasing systemic hypoxia resulting in decreased tumoral HIF-1α expression and enhancing direct effects on tumor-associated microvessels.

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