Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction

Alexandra Mikhailova, Tanja Ilmarinen, Anjula Ratnayake, G. Petrovski, Hannu Uusitalo, Heli Skottman, Mehrdad Rafat

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Corneal epithelium is renewed by limbal epithelial stem cells (LESCs), a type of tissue-specific stem cells located in the limbal palisades of Vogt at the corneo-scleral junction. Acute trauma or inflammatory disorders of the ocular surface can destroy these stem cells, leading to limbal stem cell deficiency (LSCD) - a painful and vision-threatening condition. Treating these disorders is often challenging and complex, especially in bilateral cases with extensive damage. Human pluripotent stem cells (hPSCs) provide new opportunities for corneal reconstruction using cell-based therapy. Here, we investigated the use of hPSC-derived LESC-like cells on bioengineered collagen matrices in serum-free conditions, aiming for clinical applications to reconstruct the corneal epithelium and partially replace the damaged stroma. Differentiation of hPSCs towards LESC-like cells was directed using small-molecule induction followed by maturation in corneal epithelium culture medium. After four to five weeks of culture, differentiated cells were seeded onto bioengineered matrices fabricated as transparent membranes of uniform thickness, using medical-grade porcine collagen type I and a hybrid cross-linking technology. The bioengineered matrices were fully transparent, with high water content and swelling capacity, and parallel lamellar microstructure. Cell proliferation of hPSC-LESCs was significantly higher on bioengineered matrices than on collagen-coated control wells after two weeks of culture, and LESC markers p63 and cytokeratin 15, along with proliferation marker Ki67 were expressed even after 30 days in culture. Overall, hPSC-LESCs retained their capacity to self-renew and proliferate, but were also able to terminally differentiate upon stimulation, as suggested by protein expression of cytokeratins 3 and 12. We propose the use of bioengineered collagen matrices as carriers for the clinically-relevant hPSC-derived LESC-like cells, as a novel tissue engineering approach for corneal reconstruction.

Original languageEnglish
Pages (from-to)26-34
Number of pages9
JournalExperimental Eye Research
Volume146
DOIs
Publication statusPublished - May 1 2016

Fingerprint

Pluripotent Stem Cells
Stem Cells
Epithelial Cells
Corneal Epithelium
Collagen
Keratin-12
Keratin-15
Keratin-3
Tissue Engineering
Cell- and Tissue-Based Therapy
Collagen Type I
Culture Media
Swine
Cell Culture Techniques
Cell Proliferation
Technology

Keywords

  • Corneal epithelium
  • Human pluripotent stem cells
  • Limbal epithelial stem cells
  • Regenerative medicine
  • Serum-free culture conditions
  • Tissue engineering

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction. / Mikhailova, Alexandra; Ilmarinen, Tanja; Ratnayake, Anjula; Petrovski, G.; Uusitalo, Hannu; Skottman, Heli; Rafat, Mehrdad.

In: Experimental Eye Research, Vol. 146, 01.05.2016, p. 26-34.

Research output: Contribution to journalArticle

Mikhailova, Alexandra ; Ilmarinen, Tanja ; Ratnayake, Anjula ; Petrovski, G. ; Uusitalo, Hannu ; Skottman, Heli ; Rafat, Mehrdad. / Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction. In: Experimental Eye Research. 2016 ; Vol. 146. pp. 26-34.
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