Human papillomavirus type 18 E6 mRNA in primary tumors and pelvic lymph nodes of Hungarian patients with squamous cervical cancer

J. Czegledy, M. Evander, Z. Hernádi, L. Gergely, G. Wadell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Seven biopsy specimens from squamous-cell carcinomas of the uterine cervix were examined by RT-PCR for human-papilloma-virus(HPV)-specific transcripts. With our HPV18-transcription-specific primer pair (5' nts 127-149; 3' nts 587-607), all 7 were shown to contain one strong viral mRNA signal from the early 6/early 7 open reading frames (E6/E7 ORFs). Sequence analysis of the cloned PCR product proved that the transcript was generated by splicing out an intron in E6 from nucleotides 233 to 416, thereby corresponding to the HPV18 E6 spliced mRNA. Nine out of 9 metastatic and 5 of 7 histologically negative lymph nodes from the same patients were also found to be positive for the same mRNA transcript. However, 4 HPV18 unrelated primary tumors and the connected regional pelvic lymph nodes (3 metastatic, 7 histologically negative) were negative for the HPV18 E6 mRNA. Cytokeratin signals indicating tumor cells of epithelial origin were detected in 7 out of the 9 transcript- positive lymph nodes with histological signs of metastasis and in 2 out of the 5 transcript-positive histologically negative lymph nodes. This suggests that the dispersion of the epithelial monoclonal tumor cells was lymphogenic in origin.

Original languageEnglish
Pages (from-to)182-186
Number of pages5
JournalInternational Journal of Cancer
Volume56
Issue number2
Publication statusPublished - 1994

Fingerprint

Human papillomavirus 18
Uterine Cervical Neoplasms
Lymph Nodes
Messenger RNA
Papillomaviridae
Neoplasms
Polymerase Chain Reaction
Keratins
Cervix Uteri
Introns
Open Reading Frames
Sequence Analysis
Squamous Cell Carcinoma
Nucleotides
Epithelial Cells
Neoplasm Metastasis
Biopsy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Human papillomavirus type 18 E6 mRNA in primary tumors and pelvic lymph nodes of Hungarian patients with squamous cervical cancer. / Czegledy, J.; Evander, M.; Hernádi, Z.; Gergely, L.; Wadell, G.

In: International Journal of Cancer, Vol. 56, No. 2, 1994, p. 182-186.

Research output: Contribution to journalArticle

@article{35986503f1aa4b7b848e6da39bdd595f,
title = "Human papillomavirus type 18 E6 mRNA in primary tumors and pelvic lymph nodes of Hungarian patients with squamous cervical cancer",
abstract = "Seven biopsy specimens from squamous-cell carcinomas of the uterine cervix were examined by RT-PCR for human-papilloma-virus(HPV)-specific transcripts. With our HPV18-transcription-specific primer pair (5' nts 127-149; 3' nts 587-607), all 7 were shown to contain one strong viral mRNA signal from the early 6/early 7 open reading frames (E6/E7 ORFs). Sequence analysis of the cloned PCR product proved that the transcript was generated by splicing out an intron in E6 from nucleotides 233 to 416, thereby corresponding to the HPV18 E6 spliced mRNA. Nine out of 9 metastatic and 5 of 7 histologically negative lymph nodes from the same patients were also found to be positive for the same mRNA transcript. However, 4 HPV18 unrelated primary tumors and the connected regional pelvic lymph nodes (3 metastatic, 7 histologically negative) were negative for the HPV18 E6 mRNA. Cytokeratin signals indicating tumor cells of epithelial origin were detected in 7 out of the 9 transcript- positive lymph nodes with histological signs of metastasis and in 2 out of the 5 transcript-positive histologically negative lymph nodes. This suggests that the dispersion of the epithelial monoclonal tumor cells was lymphogenic in origin.",
author = "J. Czegledy and M. Evander and Z. Hern{\'a}di and L. Gergely and G. Wadell",
year = "1994",
language = "English",
volume = "56",
pages = "182--186",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Human papillomavirus type 18 E6 mRNA in primary tumors and pelvic lymph nodes of Hungarian patients with squamous cervical cancer

AU - Czegledy, J.

AU - Evander, M.

AU - Hernádi, Z.

AU - Gergely, L.

AU - Wadell, G.

PY - 1994

Y1 - 1994

N2 - Seven biopsy specimens from squamous-cell carcinomas of the uterine cervix were examined by RT-PCR for human-papilloma-virus(HPV)-specific transcripts. With our HPV18-transcription-specific primer pair (5' nts 127-149; 3' nts 587-607), all 7 were shown to contain one strong viral mRNA signal from the early 6/early 7 open reading frames (E6/E7 ORFs). Sequence analysis of the cloned PCR product proved that the transcript was generated by splicing out an intron in E6 from nucleotides 233 to 416, thereby corresponding to the HPV18 E6 spliced mRNA. Nine out of 9 metastatic and 5 of 7 histologically negative lymph nodes from the same patients were also found to be positive for the same mRNA transcript. However, 4 HPV18 unrelated primary tumors and the connected regional pelvic lymph nodes (3 metastatic, 7 histologically negative) were negative for the HPV18 E6 mRNA. Cytokeratin signals indicating tumor cells of epithelial origin were detected in 7 out of the 9 transcript- positive lymph nodes with histological signs of metastasis and in 2 out of the 5 transcript-positive histologically negative lymph nodes. This suggests that the dispersion of the epithelial monoclonal tumor cells was lymphogenic in origin.

AB - Seven biopsy specimens from squamous-cell carcinomas of the uterine cervix were examined by RT-PCR for human-papilloma-virus(HPV)-specific transcripts. With our HPV18-transcription-specific primer pair (5' nts 127-149; 3' nts 587-607), all 7 were shown to contain one strong viral mRNA signal from the early 6/early 7 open reading frames (E6/E7 ORFs). Sequence analysis of the cloned PCR product proved that the transcript was generated by splicing out an intron in E6 from nucleotides 233 to 416, thereby corresponding to the HPV18 E6 spliced mRNA. Nine out of 9 metastatic and 5 of 7 histologically negative lymph nodes from the same patients were also found to be positive for the same mRNA transcript. However, 4 HPV18 unrelated primary tumors and the connected regional pelvic lymph nodes (3 metastatic, 7 histologically negative) were negative for the HPV18 E6 mRNA. Cytokeratin signals indicating tumor cells of epithelial origin were detected in 7 out of the 9 transcript- positive lymph nodes with histological signs of metastasis and in 2 out of the 5 transcript-positive histologically negative lymph nodes. This suggests that the dispersion of the epithelial monoclonal tumor cells was lymphogenic in origin.

UR - http://www.scopus.com/inward/record.url?scp=0028299590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028299590&partnerID=8YFLogxK

M3 - Article

C2 - 7508888

AN - SCOPUS:0028299590

VL - 56

SP - 182

EP - 186

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 2

ER -