Human oocyte vitrification: In-vivo and in-vitro maturation outcomes

Ching Chien Chang, Daniel B. Shapiro, Diana Patricia Bernal, Graham Wright, Hilton I. Kort, P. Nagy

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study aimed to evaluate oocyte vitrification efficiency using in-vivo matured (IVO) versus rescued in-vitro matured (IVM) oocytes. The results show that oocyte survival (85% versus 81%), fertilization (86% versus 76%) and cleavage rate (98% versus 89%) was not significantly different in IVO oocytes compared with rescued IVM sibling oocytes. The fertilized oocytes from IVO and IVM groups were cultured to blastocyst stage; however, embryo development was significantly reduced in the rescued IVM group (72% versus 15%). Embryo transfer was only performed with the embryos derived from IVO oocytes on day 5; 42 blastocysts were transfered to 18 recipients; 16 of 18 recipients had positive β-human chorionic gonadotrophin and a total of 26 fetal cardiac activities were detected in 15 recipients (implantation: 26/42, 61.9%). Ten of the 15 recipients have delivered 19 healthy babies, and the other five pregnancies are still ongoing. These data indicate that the combination of oocyte vitrification and rescued IVM not only yield a new strategy to extend the pool of total fertilizable oocytes, but also demonstrate that the efficiency of vitrified/warmed oocytes can be comparable to fresh oocytes with regard to clinical outcomes.

Original languageEnglish
Pages (from-to)684-688
Number of pages5
JournalReproductive BioMedicine Online
Volume17
Issue number5
Publication statusPublished - Nov 2008

Fingerprint

Vitrification
Oocytes
Embryo Transfer
In Vitro Techniques
Fetal Movement
Blastocyst
Chorionic Gonadotropin
Fertilization
Embryonic Development
Embryonic Structures

Keywords

  • Cryopreservation
  • In-vitro maturation
  • Oocyte
  • Vitrification

ASJC Scopus subject areas

  • Reproductive Medicine
  • Developmental Biology

Cite this

Chang, C. C., Shapiro, D. B., Bernal, D. P., Wright, G., Kort, H. I., & Nagy, P. (2008). Human oocyte vitrification: In-vivo and in-vitro maturation outcomes. Reproductive BioMedicine Online, 17(5), 684-688.

Human oocyte vitrification : In-vivo and in-vitro maturation outcomes. / Chang, Ching Chien; Shapiro, Daniel B.; Bernal, Diana Patricia; Wright, Graham; Kort, Hilton I.; Nagy, P.

In: Reproductive BioMedicine Online, Vol. 17, No. 5, 11.2008, p. 684-688.

Research output: Contribution to journalArticle

Chang, CC, Shapiro, DB, Bernal, DP, Wright, G, Kort, HI & Nagy, P 2008, 'Human oocyte vitrification: In-vivo and in-vitro maturation outcomes', Reproductive BioMedicine Online, vol. 17, no. 5, pp. 684-688.
Chang CC, Shapiro DB, Bernal DP, Wright G, Kort HI, Nagy P. Human oocyte vitrification: In-vivo and in-vitro maturation outcomes. Reproductive BioMedicine Online. 2008 Nov;17(5):684-688.
Chang, Ching Chien ; Shapiro, Daniel B. ; Bernal, Diana Patricia ; Wright, Graham ; Kort, Hilton I. ; Nagy, P. / Human oocyte vitrification : In-vivo and in-vitro maturation outcomes. In: Reproductive BioMedicine Online. 2008 ; Vol. 17, No. 5. pp. 684-688.
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