Human monocyte IL-10 production is increased by acute ethanol treatment

Pranoti Mandrekar, Donna Catalano, Linda Girouard, Gyongyi Szabo

Research output: Contribution to journalArticle

64 Citations (Scopus)


Immune alterations after acute ethanol treatment are characterized by abnormal monocyte mediator production and antigen presentation capacity. Here, we tested the hypothesis that some of the regulatory effects of ethanol on monocyte functions are mediated by elevated M∅ IL-10 production. Physiologically relevant in vitro doses of ethanol (25-100 mM) resulted in significantly increased IL-10 secretion by normal blood monocytes after 18 h stimulation. We found that monocyte IL-10 production induced by either ethanol or LPS increased at 10 h, maximized at 18 h and decreased by 10 h post-stimulation. Furthermore, ethanol significantly augmented LPS-induced monocyte IL-10 secretion at 18 h. Data also show that ethanol-induced changes in monocyte IL-10 mRNA levels mirror those seen at the protein levels. Greater IL-10 levels and IL-10 induction by LPS in adherent compared to non-adherent M∅ imply that adherence is an important co-stimulator for IL-10 production in human M∅. We further showed that cyclooxygenase inhibitor treatment augments M∅ IL-10 production suggesting that elevated PGE2 (and cAMP) is not necessary for IL-10 induction by ethanol or LPS in isolated M∅. Finally, our data demonstrate that ethanol-induced elevated M∅ IL-10 contributes to the decreased M∅ TNF-α production seen after acute ethanol treatment. However, observation of an ethanol-induced decrease in TNF-α mRNA as early as 1.5 h after stimulation indicate that ethanol has an additional, IL-10 independent, effect on M∅ TNF-α production. These results suggest that elevated monocyte-derived IL-10 can contribute to the monocyte as well as other immune abnormalities after acute ethanol uptake.

Original languageEnglish
Pages (from-to)567-577
Number of pages11
Issue number7
Publication statusPublished - Jul 1996


  • Adherence
  • Cyclic amp
  • Macrophages
  • PGE
  • TNF-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Human monocyte IL-10 production is increased by acute ethanol treatment'. Together they form a unique fingerprint.

  • Cite this