Human macrophages degrade tryptophan upon induction by interferon-gamma

Ernst R. Werner, Gabriele Bitterlich, Dietmar Fuchs, Arno Hausen, Gilbert Reibnegger, G. Szabó, Manfred P. Dierich, Helmut Wachter

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Human peripheral blood mononuclear cells, monocytes-macrophages and T-cells were stimulated with human recombinant interferon-gamma, interferon-alpha and phytohemagglutinin. The culture supernatants were analyzed for tryptophan, kynurenine, 3-hydroxyanthranilic acid, anthranilic acid and neopterin by high performance liquid chromatography. Tryptophan was decreased and the four other compounds were increased in supernatants of peripheral blood mononuclear cells activated by interferon-gamma (250 U/ml), interferon-alpha (10.000 U/ml) and phytohemagglutinin (1 μg/ml). After splitting of peripheral blood mononuclear cells by adherence, the monocytes and macrophages but not the T-cells degraded tryptophan upon stimulation by interferon-gamma in a dose dependent manner. Supernatants of phytohemagglutinin stimulated but not of resting T-cells were found to induce tryptophan degradation by macrophages, the active principle being neutralized by an antiserum for interferon-gamma. Thus phytohemagglutinin acts by activating T-cells to release interferon-gamma which in turn induces macrophages to degrade tryptophan. In all experiments the appearance of neopterin in the culture media was correlated to the observed tryptophan degradation.

Original languageEnglish
Pages (from-to)273-280
Number of pages8
JournalLife Sciences
Volume41
Issue number3
DOIs
Publication statusPublished - Jul 20 1987

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Macrophages
Tryptophan
Interferon-gamma
T-cells
Phytohemagglutinins
Neopterin
T-Lymphocytes
Blood Cells
Blood
Interferon-alpha
Monocytes
3-Hydroxyanthranilic Acid
Kynurenine
Degradation
High performance liquid chromatography
Culture Media
Immune Sera
High Pressure Liquid Chromatography
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Werner, E. R., Bitterlich, G., Fuchs, D., Hausen, A., Reibnegger, G., Szabó, G., ... Wachter, H. (1987). Human macrophages degrade tryptophan upon induction by interferon-gamma. Life Sciences, 41(3), 273-280. https://doi.org/10.1016/0024-3205(87)90149-4

Human macrophages degrade tryptophan upon induction by interferon-gamma. / Werner, Ernst R.; Bitterlich, Gabriele; Fuchs, Dietmar; Hausen, Arno; Reibnegger, Gilbert; Szabó, G.; Dierich, Manfred P.; Wachter, Helmut.

In: Life Sciences, Vol. 41, No. 3, 20.07.1987, p. 273-280.

Research output: Contribution to journalArticle

Werner, ER, Bitterlich, G, Fuchs, D, Hausen, A, Reibnegger, G, Szabó, G, Dierich, MP & Wachter, H 1987, 'Human macrophages degrade tryptophan upon induction by interferon-gamma', Life Sciences, vol. 41, no. 3, pp. 273-280. https://doi.org/10.1016/0024-3205(87)90149-4
Werner ER, Bitterlich G, Fuchs D, Hausen A, Reibnegger G, Szabó G et al. Human macrophages degrade tryptophan upon induction by interferon-gamma. Life Sciences. 1987 Jul 20;41(3):273-280. https://doi.org/10.1016/0024-3205(87)90149-4
Werner, Ernst R. ; Bitterlich, Gabriele ; Fuchs, Dietmar ; Hausen, Arno ; Reibnegger, Gilbert ; Szabó, G. ; Dierich, Manfred P. ; Wachter, Helmut. / Human macrophages degrade tryptophan upon induction by interferon-gamma. In: Life Sciences. 1987 ; Vol. 41, No. 3. pp. 273-280.
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