Human immunodeficiency virus type 1 tat gene enhances human cytomegalovirus gene expression and viral replication

W. Z. Ho, V. Ayyavoo, A. Srinivasan, M. F. Stinski, S. A. Plotkin, E. Gönczöl

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Clonal lines of human rhabdomyosarcoma (RD) cells, constitutively expressing human immunodeficiency virus type 1 (HIV-1) tat gene (RD tat cell lines) showed enhanced expression of human cytomegalovirus (HCMV) immediate-early (IE) and late (L) proteins upon HCMV infection, as compared with control RD cells. One of the RD tat cell lines produced infectious HCMV. The RD-tat cell lines, following transfection with recombinant plasmids containing the full length of the HCMV-IE enhancer/promoter linked to the bacterial chloramphenicol acetyltransferase (CAT) gene, exhibited an increased CAT expression by the tat product. A chronically HIV-1-infected human T-lymphoid cell line, SupT1, superinfected with HCMV, expressed HCMV-IE proteins while the parental Sup T1 cells infected with HCMV were negative. Parental Sup T1 cells coinfected with HIV-1 and HCMV also expressed HCMV-IE proteins, indicating that HIV-1-encoded proteins exert a positive regulatory effect on HCMV expression.

Original languageEnglish
Pages (from-to)689-695
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume7
Issue number8
Publication statusPublished - 1991

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tat Genes
Cytomegalovirus
HIV-1
Gene Expression
Rhabdomyosarcoma
Cell Line
Chloramphenicol O-Acetyltransferase
Cytomegalovirus Infections

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Human immunodeficiency virus type 1 tat gene enhances human cytomegalovirus gene expression and viral replication. / Ho, W. Z.; Ayyavoo, V.; Srinivasan, A.; Stinski, M. F.; Plotkin, S. A.; Gönczöl, E.

In: AIDS Research and Human Retroviruses, Vol. 7, No. 8, 1991, p. 689-695.

Research output: Contribution to journalArticle

Ho, W. Z. ; Ayyavoo, V. ; Srinivasan, A. ; Stinski, M. F. ; Plotkin, S. A. ; Gönczöl, E. / Human immunodeficiency virus type 1 tat gene enhances human cytomegalovirus gene expression and viral replication. In: AIDS Research and Human Retroviruses. 1991 ; Vol. 7, No. 8. pp. 689-695.
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