Human fetal hepatocytes respond to inflammatory mediators and excrete bile

Joachim Bauer, G. Lengyel, Swan N. Thung, Uwe Jonas, Wolfgang Gerok, George Acs

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Under strict observation of the ethical guide-lines of the 1975 Declaration of Helsinki Human Research Committee, primary hepatocyte cultures were prepared from second-trimester fetal liver specimens. We have shown for the first time that fetal hepatocytes have the capacity to produce an acute-phase response on treatment with inflammatory mediators. Addition of interleukin-6 to the cultures resulted in strong induction of C-reactive protein and α1-antichymotrypsin expression, whereas albumin expression was repressed. In contrast to interleukin-6, transforming growth factor-β did not induce C-reactive protein expression. However, as in adult hepatocytes, fetal cells responded to transforming growth factor-β by reduced albumin synthesis. We were able to show by virtue of fluorescein excretion into sealed clefts that fetal hepatocytes have the functional capacity to form bile. Our findings indicate that second-trimester hepatocytes can be regarded as fairly mature liver cells.

Original languageEnglish
Pages (from-to)1131-1141
Number of pages11
JournalHepatology
Volume13
Issue number6
Publication statusPublished - Jun 1991

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Bile
Hepatocytes
Transforming Growth Factors
Second Pregnancy Trimester
C-Reactive Protein
Albumins
Interleukin-6
Helsinki Declaration
Acute-Phase Reaction
Liver
Fluorescein
Observation
Research

ASJC Scopus subject areas

  • Hepatology

Cite this

Bauer, J., Lengyel, G., Thung, S. N., Jonas, U., Gerok, W., & Acs, G. (1991). Human fetal hepatocytes respond to inflammatory mediators and excrete bile. Hepatology, 13(6), 1131-1141.

Human fetal hepatocytes respond to inflammatory mediators and excrete bile. / Bauer, Joachim; Lengyel, G.; Thung, Swan N.; Jonas, Uwe; Gerok, Wolfgang; Acs, George.

In: Hepatology, Vol. 13, No. 6, 06.1991, p. 1131-1141.

Research output: Contribution to journalArticle

Bauer, J, Lengyel, G, Thung, SN, Jonas, U, Gerok, W & Acs, G 1991, 'Human fetal hepatocytes respond to inflammatory mediators and excrete bile', Hepatology, vol. 13, no. 6, pp. 1131-1141.
Bauer J, Lengyel G, Thung SN, Jonas U, Gerok W, Acs G. Human fetal hepatocytes respond to inflammatory mediators and excrete bile. Hepatology. 1991 Jun;13(6):1131-1141.
Bauer, Joachim ; Lengyel, G. ; Thung, Swan N. ; Jonas, Uwe ; Gerok, Wolfgang ; Acs, George. / Human fetal hepatocytes respond to inflammatory mediators and excrete bile. In: Hepatology. 1991 ; Vol. 13, No. 6. pp. 1131-1141.
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