Human epithelial tissue culture study on restorative materials

András Forster, Krisztina Ungvári, Ágnes Györgyey, Á. Kukovecz, Kinga Turzó, K. Nagy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives Health condition of the gingival tissues contacting the surfaces of fixed prostheses is a result of multiple etiologic factors. The aim of the investigation discussed here was to evaluate the attachment and proliferation rate of cultured human epithelial cells on three commonly used restorative materials under in vitro conditions. Methods Morphological and chemical structure of polished lithium-disilicate (IPS e.max Press, Ivoclar Vivadent AG, Germany), yttrium modified zirconium dioxide (5-TEC ICE Zirkon Translucent, Zirkonzahn GmbH Srl, Germany) and cobalt chromium alloy (Remanium star, Dentaurum GmbH & Co. KG, Germany) discs were examined by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and atomic force microscopy (AFM). Human epithelial cells harvested and cultured from one donor, were applied to investigate cell attachment (24 h observation) and proliferation (72 h observation) via dimethylthiazol-diphenyl tetrazolium bromide (MTT) and AlamarBlue® (AB) assays on control surface (cell-culture plate) and on the restorative materials (n = 3 × 20 specimens/material). Results SEM and AFM revealed typical morphology and roughness features for the materials. Zirconia presented significantly higher Ra value. EDS confirmed typical elements on the investigated restorative materials: lithium-disilicate (Si, O); Zirconia (Zi, Y, O); CoCr (Co, Cr, W). All surfaces except CoCr exhibited significant cell proliferation according to MTT and AB assays after 72 h compared to 24 h. Among the restorative materials, CoCr samples showed the highest cell attachment as indicated by MTT assay. AB results showed that attachment and proliferation of human epithelial cells is supported more on lithium-disilicate. Both assays indicated the lowest value for zirconia. Conclusions The results indicate that the restorative materials examined are equally suitable for subgingival restorations. Lithium-disilicate exhibited the best biocompatibility. Clinical significance The examined materials are indicated for use in restorative procedures, directly contacting the sulcular epithelial tissues. Thus it is essential to monitor the biological acceptibility of these materials in order to better understand their clinical properties. The results indicate that Lithium-disilicate is a suitable material for such purposes.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalJournal of Dentistry
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Epithelium
Germany
Atomic Force Microscopy
Epithelial Cells
Electron Scanning Microscopy
Chromium Alloys
Observation
X-Ray Emission Spectrometry
Yttrium
Bromides
Prostheses and Implants
Cell Culture Techniques
Cell Proliferation
lithia disilicate
zirconium oxide
Health
resazurin

Keywords

  • AlamarBlue assay
  • Cobalt chromium alloy
  • Human epithelial cell culture
  • Lithium-disilicate
  • MTT assay
  • Zirconia

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Human epithelial tissue culture study on restorative materials. / Forster, András; Ungvári, Krisztina; Györgyey, Ágnes; Kukovecz, Á.; Turzó, Kinga; Nagy, K.

In: Journal of Dentistry, Vol. 42, No. 1, 01.2014, p. 7-14.

Research output: Contribution to journalArticle

Forster, András ; Ungvári, Krisztina ; Györgyey, Ágnes ; Kukovecz, Á. ; Turzó, Kinga ; Nagy, K. / Human epithelial tissue culture study on restorative materials. In: Journal of Dentistry. 2014 ; Vol. 42, No. 1. pp. 7-14.
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AU - Turzó, Kinga

AU - Nagy, K.

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N2 - Objectives Health condition of the gingival tissues contacting the surfaces of fixed prostheses is a result of multiple etiologic factors. The aim of the investigation discussed here was to evaluate the attachment and proliferation rate of cultured human epithelial cells on three commonly used restorative materials under in vitro conditions. Methods Morphological and chemical structure of polished lithium-disilicate (IPS e.max Press, Ivoclar Vivadent AG, Germany), yttrium modified zirconium dioxide (5-TEC ICE Zirkon Translucent, Zirkonzahn GmbH Srl, Germany) and cobalt chromium alloy (Remanium star, Dentaurum GmbH & Co. KG, Germany) discs were examined by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and atomic force microscopy (AFM). Human epithelial cells harvested and cultured from one donor, were applied to investigate cell attachment (24 h observation) and proliferation (72 h observation) via dimethylthiazol-diphenyl tetrazolium bromide (MTT) and AlamarBlue® (AB) assays on control surface (cell-culture plate) and on the restorative materials (n = 3 × 20 specimens/material). Results SEM and AFM revealed typical morphology and roughness features for the materials. Zirconia presented significantly higher Ra value. EDS confirmed typical elements on the investigated restorative materials: lithium-disilicate (Si, O); Zirconia (Zi, Y, O); CoCr (Co, Cr, W). All surfaces except CoCr exhibited significant cell proliferation according to MTT and AB assays after 72 h compared to 24 h. Among the restorative materials, CoCr samples showed the highest cell attachment as indicated by MTT assay. AB results showed that attachment and proliferation of human epithelial cells is supported more on lithium-disilicate. Both assays indicated the lowest value for zirconia. Conclusions The results indicate that the restorative materials examined are equally suitable for subgingival restorations. Lithium-disilicate exhibited the best biocompatibility. Clinical significance The examined materials are indicated for use in restorative procedures, directly contacting the sulcular epithelial tissues. Thus it is essential to monitor the biological acceptibility of these materials in order to better understand their clinical properties. The results indicate that Lithium-disilicate is a suitable material for such purposes.

AB - Objectives Health condition of the gingival tissues contacting the surfaces of fixed prostheses is a result of multiple etiologic factors. The aim of the investigation discussed here was to evaluate the attachment and proliferation rate of cultured human epithelial cells on three commonly used restorative materials under in vitro conditions. Methods Morphological and chemical structure of polished lithium-disilicate (IPS e.max Press, Ivoclar Vivadent AG, Germany), yttrium modified zirconium dioxide (5-TEC ICE Zirkon Translucent, Zirkonzahn GmbH Srl, Germany) and cobalt chromium alloy (Remanium star, Dentaurum GmbH & Co. KG, Germany) discs were examined by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and atomic force microscopy (AFM). Human epithelial cells harvested and cultured from one donor, were applied to investigate cell attachment (24 h observation) and proliferation (72 h observation) via dimethylthiazol-diphenyl tetrazolium bromide (MTT) and AlamarBlue® (AB) assays on control surface (cell-culture plate) and on the restorative materials (n = 3 × 20 specimens/material). Results SEM and AFM revealed typical morphology and roughness features for the materials. Zirconia presented significantly higher Ra value. EDS confirmed typical elements on the investigated restorative materials: lithium-disilicate (Si, O); Zirconia (Zi, Y, O); CoCr (Co, Cr, W). All surfaces except CoCr exhibited significant cell proliferation according to MTT and AB assays after 72 h compared to 24 h. Among the restorative materials, CoCr samples showed the highest cell attachment as indicated by MTT assay. AB results showed that attachment and proliferation of human epithelial cells is supported more on lithium-disilicate. Both assays indicated the lowest value for zirconia. Conclusions The results indicate that the restorative materials examined are equally suitable for subgingival restorations. Lithium-disilicate exhibited the best biocompatibility. Clinical significance The examined materials are indicated for use in restorative procedures, directly contacting the sulcular epithelial tissues. Thus it is essential to monitor the biological acceptibility of these materials in order to better understand their clinical properties. The results indicate that Lithium-disilicate is a suitable material for such purposes.

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