Human cytokines interleukin (il)-3 and il-6 affect the growth and insulin binding of the unicellular organismtetrahymena

G. Csaba, P. Kovács, A. Falus

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Interleukin (IL)-3 and IL-6 significantly increase the growth rate of the unicellular organism, Tetrahymena. The effect elicited by IL-3 is long lasting as it was also detectable after 20 generations. Effect of IL-6 was detectable as long as the substance was present in the cell culture. Pretreatment with IL-3 did not enhance the proliferative response to subsequent IL-3 treatment, but the second exposure to IL-3 considerably depressed the active proliferation ofTetrahymenacells. However, a positive ‗priming effect‘ elicited by IL-6 resulted in an increased growth rate following repeated IL-6 stimulation. Insulin binding to the plasma membrane ofTetrahymenawas increased by IL-6 but not by IL-3 after 24 hours, and this enhancement appeared even after one hour incubation. If the cells were pretreated with insulin, IL-6 did not influence insulin binding, while an inhibition by IL-3 was observed. These results direct attention to the similarities of actions induced by IL-3 and IL-6 at different levels of phylogeny probably due to the presence of cytokine receptor-like structures on this unicellular organism.

Original languageEnglish
Pages (from-to)771-774
Number of pages4
JournalCytokine
Volume7
Issue number8
DOIs
Publication statusPublished - Nov 1995

Keywords

  • Il-3
  • Il-6
  • Insulin binding
  • Tetrahymena

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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