The unicellular ciliate, Tetrahymena contains molecules which immunologically and functionally resemble to human hormones. Up to now, of the pituitary glycoprotein hormones only the presence of adrenocorticotropic hormone (ACTH) was demonstrated. In the present experiment we observed the presence of molecules immunologically similar to human chorionic gonadotropin (HCG). This hormone-like material is localized diffusely in the cell, and in higher amount in the cortical region, in the cilia and in the oral field. After one hour treatment (hormonal imprinting) with luteinizing hormone (LH), follicle stimulating hormone, mixture of these two hormones, HCG, or thyrotropic hormone (TSH), more fluorescence intensity (given by the hormone) can be observed. Twenty four hours or four days after these treatments the amount of hormone-like materials seems to be higher, than in the untreated cells or immediately after treatment. The diffuse localization disappears in the body of the cell after LH or FSH treatment and the hormone concentrates peripheral, in the cortex and especially in (or on) the cilia. The largest amount of hormone-like material seems to be present after LH+FSH or TSH treatment. These results mean that in Tetrahymena enhanced production of the appropriate hormone could be induced by hormone treatment (imprinting), which is maintained in the progeny generations. The experiments support that hormonal imprinting of receptor and hormone production can run parallel.
|Number of pages||8|
|Publication status||Published - Jan 1 2000|
- Hormonal imprinting
- Pituitary hormones
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)