Human cationic trypsinogen is sulfated on Tyr154

Miklós Sahin-Tóth, Z. Kukor, Z. Nemoda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The crystal structure of human pancreatic cationic trypsin showed the chemical modification of Tyr154, which was originally described as phosphorylation [Gaboriaud C, Serre L, Guy-Crotte O, Forest E & Fontecilla-Camps JC (1996) J Mol Biol259, 995-1010]. Here we report that Tyr154 is sulfated, not phosphorylated. Cationic and anionic trypsinogens were purified from human pancreatic juice and subjected to alkaline hydrolysis. Modified tyrosine amino acids were separated on a Dowex cation-exchange column and analyzed by thin layer chromatography. Both human cationic and anionic trypsinogens contained tyrosine sulfate, but no tyrosine phosphate, whereas bovine trypsinogen contained neither. Furthermore, incorporation of [ 35S]SO4 into human cationic trypsinogen transiently expressed by human embryonic kidney 239T cells was demonstrated. Mutation of Tyr154 to Phe abolished radioactive sulfate incorporation, confirming that Tyr154 is the site of sulfation in cationic trypsinogen. Sulfated pancreatic cationic trypsinogen exhibited faster autoactivation than a nonsulfated recombinant form, suggesting that tyrosine sulfation of trypsinogens might enhance intestinal digestive zymogen activation in humans. Finally, sequence alignment revealed that the sulfation motif is only conserved in primate trypsinogens, suggesting that typsinogen sulfation is absent in other vertebrates.

Original languageEnglish
Pages (from-to)5044-5050
Number of pages7
JournalFEBS Journal
Volume273
Issue number22
DOIs
Publication statusPublished - Nov 2006

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Trypsinogen
Tyrosine
Pancreatic Juice
Thin layer chromatography
Enzyme Precursors
Phosphorylation
Sequence Alignment
Chemical modification
Thin Layer Chromatography
Trypsin
Primates
Sulfates
Vertebrates
Cations
Hydrolysis
Crystal structure
Chemical activation
Phosphates
Kidney
Amino Acids

Keywords

  • Hereditary pancreatitis
  • Human anionic trypsin
  • Human cationic trypsin
  • Post-translational modification
  • Tyrosine sulfation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Human cationic trypsinogen is sulfated on Tyr154. / Sahin-Tóth, Miklós; Kukor, Z.; Nemoda, Z.

In: FEBS Journal, Vol. 273, No. 22, 11.2006, p. 5044-5050.

Research output: Contribution to journalArticle

Sahin-Tóth, Miklós ; Kukor, Z. ; Nemoda, Z. / Human cationic trypsinogen is sulfated on Tyr154. In: FEBS Journal. 2006 ; Vol. 273, No. 22. pp. 5044-5050.
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