Human astroviruses in raw sewage samples in Hungary

E. Meleg, F. Jakab, B. Kocsis, K. Bányai, B. Melegh, G. Szucs

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Aims: Routine procedures for monitoring viruses in water samples have not been drawn up for the water-microbiology screening panel. Enteric viruses, including astroviruses, are able to persist under environmental conditions and may cause public health problems by contaminating natural and drinking water resources. The aim of this study was to detect human astroviruses (HAstVs) from raw wastewater samples. Methods and Results: To obtain data on whether human astroviruses are shed in the environment, 35 raw sewage samples from 22 sewage plants in different regions of Baranya County, Hungary were tested for astrovirus using a polyethylene glycol method for concentration and a guanidinium thiocyanate-silica procedure for extraction of viral RNA. Reverse transcription-polymerase chain reaction (RT-PCR) with HAstV-specific primer pairs was used for amplification and the specificity of amplicons was confirmed by nucleotide sequencing and phylogenetic analysis. Among the 35 raw sewage samples, 15 (43%) contained HAstV and by sequence analysis, 10 genotype HAstV-1 and one genotype HAstV-2 were identified. Conclusions: The high detection rate of astroviruses we encountered in this study provide convincing evidence that HAstVs circulate at a relatively high frequency in the Hungarian population. No correlation between the standard indicators of faecal pollution and the presence of HAstVs was found. Significance and Impact of the Study: Our study is the first report on detection of HAstV in sewage in Hungary and suggests that HAstV might be potent indicators of viral pollution in environmental specimens.

Original languageEnglish
Pages (from-to)1123-1129
Number of pages7
JournalJournal of Applied Microbiology
Volume101
Issue number5
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Human astrovirus
Mamastrovirus
Hungary
Sewage
sewage
Water Microbiology
Astroviridae
Genotype
Water Resources
sampling
Environmental Pollution
Enterovirus
Viral RNA
Waste Water
Silicon Dioxide
Drinking Water
Reverse Transcription
Sequence Analysis
Nucleotides
Public Health

Keywords

  • Astroviruses
  • Guanidinium thiocyanate
  • Polyethylene glycol
  • RT-PCR
  • Sewage

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Human astroviruses in raw sewage samples in Hungary. / Meleg, E.; Jakab, F.; Kocsis, B.; Bányai, K.; Melegh, B.; Szucs, G.

In: Journal of Applied Microbiology, Vol. 101, No. 5, 11.2006, p. 1123-1129.

Research output: Contribution to journalArticle

Meleg, E. ; Jakab, F. ; Kocsis, B. ; Bányai, K. ; Melegh, B. ; Szucs, G. / Human astroviruses in raw sewage samples in Hungary. In: Journal of Applied Microbiology. 2006 ; Vol. 101, No. 5. pp. 1123-1129.
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