Human adult epidermal melanocytes cultured without chemical mitogens express the EGF receptor and respond to EGF

Gábor Szabad, Bernadett Kormos, Andor Pivarcsi, Márta Széll, Kornélia Kis, Anna Kenderessy Szabó, Attila Dobozy, Lajos Kemény, Zsuzsanna Bata-Csörgő

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8 Citations (Scopus)

Abstract

We describe a novel chemical mitogen-free in vitro culture technique for obtaining pure melanocyte cultures using normal human adult epidermis as a source. The culture medium consists equal parts of the commercially available Keratinocyte Basal and AIM-V media (both from Gibco), as basal medium, which is supplemented with fetal bovine serum, bovine pituitary extract and recombinant human epidermal growth factor (EGF). Melanocytes harvested from human adult skin proliferate extensively and can be passaged serially up to 10-15 times using this medium. We have verified the identity of the cultured cells by tyrosinase mRNA expression and TRP-1 protein staining. Moreover, we showed that autologous human serum alone, without additional supplements is able to provide sufficient growth support for the cultured cells in the basal medium, making this culture technique suitable for autologous melanocyte transplantation. In this culture system normal human adult melanocytes expressed both EGF receptor (EGFR) mRNA and protein and EGF showed a dose dependent mitogenic effect on the cells. EGF itself had no significant influence on EGFR mRNA expression.

Original languageEnglish
Pages (from-to)191-200
Number of pages10
JournalArchives of Dermatological Research
Volume299
Issue number4
DOIs
Publication statusPublished - Jul 2007

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Keywords

  • EGF
  • EGF receptor
  • Melanocytes
  • Proliferation

ASJC Scopus subject areas

  • Dermatology

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