hsp47 and hsp70 Gene expression is differentially regulated in a stress- and tissue-specific manner in zebrafish embryos

Zsolt Lele, Shane Engel, Patrick H. Krone

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

We nave examined differences in the spatial and tempora regulation of stress-induced hsp47 and hsp70 gene expression following exposure of zebrafish embryos to heat shock or ethanol. Using Northern blot analysis, we found that levels of hsp47 and hsp70 mRNA were dramatically elevated during heat shock in 2-day-old embryos. In contrast ethanol exposure resulted in strong upregulation of the hsp47 gene whereas hsp70 mRNA levels increased only slighty following the same treatment. Whole-mount in situ hybridization analysis revealed thai hsp47 mRNA was expressed predominantly in precartilagenous cells as well as several other connective tissue cel populations within the embryo following exposure to either stress. hsp70 mRNA displayed a very different cell-specific distribution. For example, neither stress reduced hsp70 mRNA accumulation in precartilogenous cells. However, high levels of hsp70 mRNA were detectable in epithelial cells of me developing epidermis following exposure to heat shock, but not to ethanol. These cells did not express me hsp47 gene following exposure to elmer of these stresses. The results suggest the presence of different inducible regulatory mechanisms for these genes which operate in a cell- and stress- specific manner in zebrafish embryos.

Original languageEnglish
Pages (from-to)123-133
Number of pages11
JournalDevelopmental Genetics
Volume21
Issue number2
DOIs
Publication statusPublished - Oct 16 1997

Keywords

  • Ethanol
  • Heat shock
  • Zebrafish
  • hsp47
  • hsp70

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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