HPTLC and HPLC determination of alkaloids in poppies subjected to stress

Beáta Szabó, Ágnes Lakatos, Tamás Koszegi, Lajos Botz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Papaver somniferum produces secondary metabolites which have important roles in self-defense processes, in plant biochemistry, and in allelochemistry. By performing experiments to determine how irregular stress effects changed the alkaloid content of poppies we have shown that different types of stress affect the quantities of alkaloids. Papaver somniferum (cv. 'Kék Duna', Budakalász, Hungary) plants were grown for 2 months, from seeds, in quartzsand (natural light, temperature 24-28°C, in Knopf's nutritive solution). In this work we studied alkaloids in poppies subjected to two kinds of stress - mycotoxin and drought. Amounts of alkaloids were measured by different separation and detection procedures - thin-layer chromatography (TLC and HPTLC) with fluorescence detection, and high-performance liquid chromatography (HPLC). HPLC proved superior for identification and approximate estimation of the morphine alkaloids, but the effects of stress on poppy plants can be detected by use of either method. Drought stress resulted in higher levels of the alkaloids whereas mycotoxin stress did not result in significant differences.

Original languageEnglish
Pages (from-to)293-297
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume16
Issue number4
DOIs
Publication statusPublished - Jul 1 2003

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Keywords

  • HPLC
  • Morphine alkaloids
  • Stress effects
  • TLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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