HPLC–MS/MS-based metabolic profiling and pharmacological properties of extracts and infusion obtained from Amelanchier parviflora var. dentata

Gokhan Zengin, Alina Diuzheva, J. Jekó́, Zoltán Cziáky, Gizem Bulut, Ahmet Dogan, Mehmet Zeki Haznedaroglu, Kannan R.R. Rengasamy, Devina Lobine, Mir Babak Bahadori, Mohamad Fawzi Mahomoodally

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4 Citations (Scopus)

Abstract

Amelanchier parviflora var. dentata is an endemic medicinal plant traditionally consumed to treat stomach pain, cardiovascular diseases, and urinary tract infections in Turkey's folk medicine. Although Amelanchier genus is an excellent source of phytochemicals with a panoply of biological activities, no report has been made available on Amelanchier parviflora var. dentata. Thus, the metabolic profiles of methanol, ethyl acetate, and water extracts were obtained using HPLC–MS/MS. Besides, the pharmacological properties such as antioxidant activity and enzyme inhibition effects against acetyl/butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase were evaluated. In total, 62, 38, and 16 metabolites were identified from the methanolic, water, and ethyl acetate extracts, respectively. The identified metabolites belong to anthocyanins, phenolic acids, and flavonoids. The tested extracts exhibited different enzyme inhibitory activity against cholinesterases (acetylcholinesterase: 0.52–2.47 mg GEs/g extract and butyrylcholinesterase: 0.47–3.59 mg GEs/g extract), tyrosinase (19.0–145.54 mg KAEs/g extract), α-amylase (0.23–1.10 mmol ACEs/g extract), and α-glucosidase (8.89–9.92 mmol ACEs/ g extract). Evidence from the present study supports the necessity for more research in an endeavor to discover biologically active secondary metabolites from A. parviflora to manage specific ailments.

Original languageEnglish
Pages (from-to)699-706
Number of pages8
JournalIndustrial Crops and Products
Volume124
DOIs
Publication statusPublished - Nov 15 2018

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Keywords

  • Amelanchier parviflora
  • Anthocyanins
  • Antioxidants
  • Chemical fingerprints
  • Enzyme inhibitors
  • Phenolics

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Zengin, G., Diuzheva, A., Jekó́, J., Cziáky, Z., Bulut, G., Dogan, A., Haznedaroglu, M. Z., Rengasamy, K. R. R., Lobine, D., Bahadori, M. B., & Mahomoodally, M. F. (2018). HPLC–MS/MS-based metabolic profiling and pharmacological properties of extracts and infusion obtained from Amelanchier parviflora var. dentata. Industrial Crops and Products, 124, 699-706. https://doi.org/10.1016/j.indcrop.2018.08.042