HPLC study on Fenton-reaction initiated oxidation of salicylic acid. Biological relevance of the reaction in intestinal biotransformation of salicylic acid

Eszter Nyúl, Mónika Kuzma, Mátyás Mayer, Sándor Lakatos, Attila Almási, P. Perjési

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fenton-reaction initiated in vitro oxidation and in vivo oxidative biotransformation of salicylic acid was investigated by HPLC-UV-Vis method. By means of the developed high performance liquid chromatography (HPLC) method salicylic acid, catechol, and all the possible monohydroxylated derivatives of salicylic acid can be separated. Fenton oxidations were performed in acidic medium (pH 3.0) with two reagent molar ratios: (1) salicylic acid: iron: hydrogen peroxide 1:3:1 and (2) 1:0.3:1. The incubation samples were analysed at different time points of the reactions. The biological effect of elevated reactive oxygen species concentration on the intestinal metabolism of salicylic acid was investigated by an experimental diabetic rat model. HPLC-MS analysis of the in vitro samples revealed presence of 2,3- and 2,5-dihydroxybenzoic acids. The results give evidence for nonenzyme catalysed intestinal hydroxylation of xenobiotics.

Original languageEnglish
JournalFree Radical Research
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Salicylic Acid
High performance liquid chromatography
Biotransformation
High Pressure Liquid Chromatography
Oxidation
Gentisates
Hydroxylation
Xenobiotics
Metabolism
Hydrogen Peroxide
Rats
Reactive Oxygen Species
Iron
Derivatives

Keywords

  • Diabetes
  • Fenton reaction
  • nonsteroidal anti-inflammatory agents (NSAIDs)
  • reactive oxygen species (ROS)
  • salicylic acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

HPLC study on Fenton-reaction initiated oxidation of salicylic acid. Biological relevance of the reaction in intestinal biotransformation of salicylic acid. / Nyúl, Eszter; Kuzma, Mónika; Mayer, Mátyás; Lakatos, Sándor; Almási, Attila; Perjési, P.

In: Free Radical Research, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Lakatos, Sándor

AU - Almási, Attila

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