HPLC separation of opium alkaloids on porous and non-porous stationary phases

L. Krenn, B. Boros, R. Ohmacht, L. Jelinek

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.

Original languageEnglish
JournalChromatographia
Volume51
Issue numberSUPPL. 2
Publication statusPublished - 2000

Fingerprint

Opium
Alkaloids
High Pressure Liquid Chromatography
Thebaine
Noscapine
Codeine
Papaverine
Standardization
Morphine

Keywords

  • Column liquid chromatography
  • Non-porous stationary phase
  • Opium alkaloids
  • Poppy, Papaver somniferum L.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

HPLC separation of opium alkaloids on porous and non-porous stationary phases. / Krenn, L.; Boros, B.; Ohmacht, R.; Jelinek, L.

In: Chromatographia, Vol. 51, No. SUPPL. 2, 2000.

Research output: Contribution to journalArticle

Krenn, L. ; Boros, B. ; Ohmacht, R. ; Jelinek, L. / HPLC separation of opium alkaloids on porous and non-porous stationary phases. In: Chromatographia. 2000 ; Vol. 51, No. SUPPL. 2.
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