HPLC separation of opium alkaloids on porous and non-porous stationary phases

L. Krenn, B. Boros, R. Ohmacht, L. Jelinek

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10 Citations (Scopus)


A new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.

Original languageEnglish
Pages (from-to)S175-S178
Issue number1
Publication statusPublished - Jan 1 2000



  • Column liquid chromatography
  • Non-porous stationary phase
  • Opium alkaloids
  • Poppy, Papaver somniferum L.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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