HPLC-MS analysis of sennosides A and B in aqueous extracts of sennae folium prepared by a new microwave extraction method

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3 Citations (Scopus)

Abstract

A new, simplified open-vessel microwave extraction (OVME) method with solid-phase extraction (SPE) for sample clean-up has been developed for specific investigation of sennosides A and B. Aqueous extracts of senna leaves ( Senna folium ) were prepared by different methods, including classic pharmacopoeial methods: concoction, infusion, and ultrasound extractions. The different extraction methods were compared by analysis of the sennosides A and B in the samples with a modified enantiomer-selective reversed-phase high-performance liquid chromatographic-tandem mass spectrometric method. HPLC was performed with a 4.6 mm × 150 mm, 3.5-μm particle C 8 column and simple gradient elution. The benefit of open-vessel microwave extraction is discussed.

Original languageEnglish
Pages (from-to)473-482
Number of pages10
JournalActa Chromatographica
Volume21
Issue number3
DOIs
Publication statusPublished - Sep 1 2009

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Microwaves
Senna Extract
Enantiomers
Ultrasonics
sennoside A&B
Liquids

Keywords

  • Column liquid chromatography-tandem mass spectrometry (HPLC-MS)
  • Microwave extraction
  • Senna
  • Sennosides A and B
  • Solid-phase extraction (SPE)

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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title = "HPLC-MS analysis of sennosides A and B in aqueous extracts of sennae folium prepared by a new microwave extraction method",
abstract = "A new, simplified open-vessel microwave extraction (OVME) method with solid-phase extraction (SPE) for sample clean-up has been developed for specific investigation of sennosides A and B. Aqueous extracts of senna leaves ( Senna folium ) were prepared by different methods, including classic pharmacopoeial methods: concoction, infusion, and ultrasound extractions. The different extraction methods were compared by analysis of the sennosides A and B in the samples with a modified enantiomer-selective reversed-phase high-performance liquid chromatographic-tandem mass spectrometric method. HPLC was performed with a 4.6 mm × 150 mm, 3.5-μm particle C 8 column and simple gradient elution. The benefit of open-vessel microwave extraction is discussed.",
keywords = "Column liquid chromatography-tandem mass spectrometry (HPLC-MS), Microwave extraction, Senna, Sennosides A and B, Solid-phase extraction (SPE)",
author = "{\'A} D{\'a}vid and E. Mincsovics and K. P{\'a}pai and K. Lud{\'a}nyi and I. Antal and I. Klebovich",
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T1 - HPLC-MS analysis of sennosides A and B in aqueous extracts of sennae folium prepared by a new microwave extraction method

AU - Dávid, Á

AU - Mincsovics, E.

AU - Pápai, K.

AU - Ludányi, K.

AU - Antal, I.

AU - Klebovich, I.

PY - 2009/9/1

Y1 - 2009/9/1

N2 - A new, simplified open-vessel microwave extraction (OVME) method with solid-phase extraction (SPE) for sample clean-up has been developed for specific investigation of sennosides A and B. Aqueous extracts of senna leaves ( Senna folium ) were prepared by different methods, including classic pharmacopoeial methods: concoction, infusion, and ultrasound extractions. The different extraction methods were compared by analysis of the sennosides A and B in the samples with a modified enantiomer-selective reversed-phase high-performance liquid chromatographic-tandem mass spectrometric method. HPLC was performed with a 4.6 mm × 150 mm, 3.5-μm particle C 8 column and simple gradient elution. The benefit of open-vessel microwave extraction is discussed.

AB - A new, simplified open-vessel microwave extraction (OVME) method with solid-phase extraction (SPE) for sample clean-up has been developed for specific investigation of sennosides A and B. Aqueous extracts of senna leaves ( Senna folium ) were prepared by different methods, including classic pharmacopoeial methods: concoction, infusion, and ultrasound extractions. The different extraction methods were compared by analysis of the sennosides A and B in the samples with a modified enantiomer-selective reversed-phase high-performance liquid chromatographic-tandem mass spectrometric method. HPLC was performed with a 4.6 mm × 150 mm, 3.5-μm particle C 8 column and simple gradient elution. The benefit of open-vessel microwave extraction is discussed.

KW - Column liquid chromatography-tandem mass spectrometry (HPLC-MS)

KW - Microwave extraction

KW - Senna

KW - Sennosides A and B

KW - Solid-phase extraction (SPE)

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