HPLC method for Measurement of human salivary α-Amylase inhibition by aqueous plant extracts

István Takács, Ákos Takács, A. Pósa, G. Gyémánt

Research output: Contribution to journalArticle

Abstract

Control of hyperglycemia is an important treatment in metabolic disorders such as type II diabetes and obesity. a-Amylase, as the first enzyme of glucose release from dietary polysaccharides, is a potential target to identify new sources of novel anti-obesity and anti-diabetic drugs. In this work, different herbal extracts as a-amylase inhibitors were studied by measuring the rate of the cleavage of a maltooligomer substrate 2-chloro-4-nitrophenyl-β-D-maltoheptoside. Measurement of chromophore containing products after reversed phase HPLC separation was used for a-amylase activity measurement. Rates of hydrolysis catalysed by human salivary a-amylase were determined in the presence and absence of lyophilised water extracts of eleven herbs. Remarkable bioactivities were found for extracts of Cinnamomum zeylanicum Blume (bark), Camellia sinensis L. (leaf), Ribes nigrum L. (leaf), Laurus nobilis L. (leaf), Vaccinium macrocarpon Aiton (fruit) and Syzygium aromaticum L. (bud). Determined IC50 values were in 0.017-41 (ig/ml range for these six selected plant extracts. Our results confirm the applicability of this HPLC-based method for the quick and reliable comparison of plants as a-amylase inhibitors.

Original languageEnglish
Pages (from-to)127-136
Number of pages10
JournalActa Biologica Hungarica
Volume68
Issue number2
DOIs
Publication statusPublished - Jun 1 2017

Fingerprint

plant extract
Plant Extracts
Amylases
obesity
High Pressure Liquid Chromatography
inhibitor
bioactivity
diabetes
polysaccharide
Laurus
bud
bark
cleavage
herb
Ribes
Vaccinium macrocarpon
Anti-Obesity Agents
Camellia sinensis
hydrolysis
Cinnamomum zeylanicum

Keywords

  • Diabetes
  • HPLC
  • Inhibition
  • Obesity
  • Plant extract
  • α-amylase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

HPLC method for Measurement of human salivary α-Amylase inhibition by aqueous plant extracts. / Takács, István; Takács, Ákos; Pósa, A.; Gyémánt, G.

In: Acta Biologica Hungarica, Vol. 68, No. 2, 01.06.2017, p. 127-136.

Research output: Contribution to journalArticle

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