The bonding of 11-aminoundecanoic (11-AA), 8-amino octanoic and 6-aminohexanoic acids as representatives of Zwitterionic ion-pairing agents, was determined on C18column in the pH range 3–8. The break through curve of 11 - AA has shown minimum in the vicinity of the isoelectric point. In the same pH range the chromatographic behaviour of fluoroquinolone gyrase inhibitor derivatives was studied in 0.002 M 11-AA containing methanol-phosphate buffer (1:1) eluent. The retention of the fluoroquinolones has shown a maximum close to the pH of the isoelectric points. As explanation the adsorption of the neutral form is suggested by the authors. The reverse chromatographic behaviour of 11-AA and the fluoroquinolone lomefloxacin harmonizes well with the results of pH dependent species distribution.
ASJC Scopus subject areas
- Molecular Medicine