HPLC enantioseparation of β2-homoamino acids using crown ether-based chiral stationary phase

R. Berkecz, I. Ilisz, Aleksandra Misicka, Dagmara Tymecka, F. Fülöp, Hee Jung Chol, Myung Ho Hyun, A. Péter

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

RP high-performance liquid chromatographic methods were developed for the enantioseparation of eleven unusual β2-homoamino acids. The underivatized analytes were separated on a chiral stationary phase containing (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid as chiral selector. The effects of organic (alcoholic) and acidic modifiers, the mobile phase composition and temperature on the separation were investigated. The structures of the substituents in the α-position of the analytes substantially influenced the retention and resolution. The elution sequence was determined in some cases: the S enantiomers eluted before the R enantiomers.

Original languageEnglish
Pages (from-to)981-987
Number of pages7
JournalJournal of Separation Science
Volume32
Issue number7
DOIs
Publication statusPublished - 2009

Fingerprint

Crown Ethers
Crown ethers
Enantiomers
Acids
Phase composition
Liquids
Temperature
18-crown-6 2,3,11,12-tetracarboxylic acid

Keywords

  • (+)-(18-Crown-6)-2,3,11,12-tetracarboxylic acid-based chiral stationary phase
  • B-Homoamino acids
  • Column liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

Cite this

HPLC enantioseparation of β2-homoamino acids using crown ether-based chiral stationary phase. / Berkecz, R.; Ilisz, I.; Misicka, Aleksandra; Tymecka, Dagmara; Fülöp, F.; Chol, Hee Jung; Hyun, Myung Ho; Péter, A.

In: Journal of Separation Science, Vol. 32, No. 7, 2009, p. 981-987.

Research output: Contribution to journalArticle

Berkecz, R. ; Ilisz, I. ; Misicka, Aleksandra ; Tymecka, Dagmara ; Fülöp, F. ; Chol, Hee Jung ; Hyun, Myung Ho ; Péter, A. / HPLC enantioseparation of β2-homoamino acids using crown ether-based chiral stationary phase. In: Journal of Separation Science. 2009 ; Vol. 32, No. 7. pp. 981-987.
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